Effect Of Fluctant Glucose On The Expression And Anti-inflammatory Function Of PParγ In Cultured Mice Vascular Smooth Muscle Cells

Chapter one Effect of fluctant glucose on the expression of PPARyObjective:This part, we investigated the effects of fluctant glucose on the expression of Peroxisome Proliferator-activated Receptor-y(PPARy) in cultured mice vascular smooth muscle cells (mVSMCs).Methods:Mice vascular smooth muscle cells were divided into four groups and cultured in four different culture conditions for24hours respectively:normal glucose group (NG group,5.5mmol/L Glu), high glucose group (HG group,25mmol/L Glu), glucose fluctant group(FG group,5.5mmol/L Glu for2h,25mmol/L Glu for3h,25mmol/L Glu for the whole night), mannitol group (MG group,5.5mmol/L Glu+19.5mmol/L mannitol), Real-Time PCR and Western blot were used to detect the changes in PPARy mRNA and protein expression in the cells after the treatment.Results:The expression of PPARy mRNA were significantly higher in HG and FG group than in NG group (P<0.05). However, mannitol group did not differ significantly from NG group in the expression of PPARy mRNA(P>0.05). The expression of PPARy protein was significantly higher in HG group than in NG group (P<0.05). The expression of PPARy protein was significantly higher in FG group than in HG group (P<0.05). Mannitol group did not differ significantly from NG group in the expression of PPARy protein(P>0.05).Conclusion:High glucose and fluctant glucose increased the expression of the PPARy in mice vascular smooth muscle cells, this tendency was more significant in the fluctant glucose condition. Chapter Two Effect of fluctant glucose on the anti-inflammatory function of PPARγ in cultured mice vascular smooth muscle cellsObjective:In the above study, we found fluctant glucose increased the expression of of PPARγ in mice vascular smooth muscle cells. In the present study, we investigated the effect of fluctant glucose on the anti-inflammatory function of PPARγ in cultured mice vascular smooth muscle cells.Methods:After being cultured and passaged, the VSMCs were cultured in eight different culture conditions for24hours respectively: normal glucose group (G1,5.5mmol/L Glu), normal glucose+Rosiglitazone group (G2,5.5mmol/L Glu+10μmol/L rosiglitazone), high glucose group (G3,25mmol/L Glu), high glucose+Rosiglitazone group (G4,25mmol/L Glu+10μmol/L rosiglitazone), glucose fluctant group (G5,5.5mmol/L Glu for2h,25mmol/L Glu for3h,25mmol/L Glu for the whole night), glucose fluctant+Rosiglitazone group (G6,5.5mmol/L Glu for2h,25mmol/L Glu for3h,25mmol/L Glu for the whole night+10μmol/L rosiglitazone), mannitol group (G7,5.5mmol/L Glu+19.5mmol/L mannitol), mannitol+Rosiglitazone group (G8,5.5mmol/L Glu+19.5mmol/L mannitol+10μmol/L rosiglitazone), then the expressions of MCP-1were detected by ELISA.Results:ELISA results showed that compared with the normal control group and the mannitol group, the expressions of MCP-1in high glucose group and glucose fluctant group were higher (P<0.05), and the the expression of MCP-1in glucose fluctant group was much higher than that of high glucose group(P<0.05).The rosiglitazone induced depression of the expressions of MCP-1were obvious in high glucose group and glucose fluctant group especially in glucose fluctant group (P<0.05).Conclusion:High glucose and glucose fluctation promoted MCP-1expression, the activation of PPARy by rosiglitazone could reduce MCP-1expression. This anti-inflammatory function of PPARy weakened in the high glucose and glucose fluctant situation, especially in the glucose fluctant group.6figures,1table and89references.