Correlation Between Gene Polymorphism Of MSH5C85T And The Susceptibility Of Non-small-cell Lung Carcinoma

Objective:To investigate the association of MSH5gene C85T polymorphism with the susceptibility of non-small-cell lung carcinoma (NSCLC), and to povide new evidence for studying the etiology of non-small-cell carcinoma.Methods:The MSH5gene C85T polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), randomly to select50%samples to sequence.Results:The MSH5C85T genotype frequencies of CC,CT and TT were59.8%,35.5%,4.7%in NSCLC group, and78.3%,20.0%,1.7%in normal control group, respectively. The frequency of the CT+TT genotype in NSCLC was significantly higher than that in controls (χ2is9.170, P is0.002, OR is1.855(95%CI is1.229～2.798). The frequencies of the two alleles C and T were77.6%and22.4%in NSCLC, and were88.3%and11.7%in normal control group, respectively. The T allele frequency was significantly higher in NSCLC than in controls (χ2is9.405, P is0.002, OR is1.923(95%CI is1.253～2.950). Results of sequencing were completely consistent with those obtained by PCR-RFLP method.Conclusion:Our study demonstrated MSH5C85T polymorphism is associated with susceptibility of NSCLC significantly in a Chinese population.