The Expression Of CSNK2B In Hepatocarcinoma And The Effect Of CSNK2B Specific SiRNA On The Growth Of Liver Cancer Cells

BACKGROUND AND OBJUECTIVE:CK2is called casein kinase2. It is a kind of serine/threonine kinase and is highly conservative in eukaryotic cells. CK2got multiple functions in the whole cell life. In recent years the research to CK2had made significant progress. CK2took part in the regulation of cell proliferation and cell apoptosis[1]. The research mainly involved in head and neck cancers.prostate,breast and lung cancer[2,4]. Paper showed that each subunits of CK2could function as whole enzyme or could exists freely and functional separately[2,3,5]. This study was to explore the expression and CSNK2B in liver cancer cells and to investigate their correlations to clinicopathologic features of HCC. Furthermore to assess the effect of small interfering RNA(siRNA) specific to CSNK2B on proliferation and migration of liver cancer cell7721. we test the antitumor drugs sensitivity of CSNK2B in liver cancer cells. After knocking down the CSNK2B we explore whether the sensitivity had changed when10kinds of drugs had added.Methods:through the realtime-PCR detection method,we tested the CSNK2B expression in mRNA level in30samples and100samples which got pathological data. Immunohistochemical method was used to examine the expression of CSNK2B in paraffin-embedded speciments of75liver cancer tissues and75adjacent cancer tissues with pathological data. Three specific siRNA fragments to CSNK2B was designed to knockdown the expression of CSNK2B. we transfect the cells with siRNA fragments using the non silence fragment as the control group. we test the expression of CSNK2B in both the mRNA level and protein expression level using PT-PCR and WESTERN BOLT. We draw the cell growth curve, cloning forming number and cell migration efficiency rate. We also test the drugs effect to the cell growth rate with MTT.Results:mRNA level test showed that the expression of CSNK2B in liver cancer tissue is significantly higher than the adjacent cancer organization. The positive rate is71.8%. The immunohistochemical result also shows,the positive rate in cancer tissue is87.8%while the positive rate in adjacent tissue is27.0%(p<0.01). the high expression in cancer showed both in cytoplasm and cell nucleus. The correlation analysis showed that the expression of CSNK2B is correlated with age, Hepatic cirrhotic nodule, Pathological differentiation.We test the siRNA efficiency through the both mRNA level and protein level. The results showed that1#and2#fragments can effectively knockdown the expression of the CSNK2B. We transfect the7721with small siRNA, silencing of CSNK2B reduced the cell growth rate. The colony forming rate is lower too. The scratch test showed the migration slow down when knocking down the CSNK2B. the antitumor drug testing results show that,when downregulated the CSNK2B, the cell sensitivity to dox,dau and CPT increased.Conclusions:our results suggest that CSNK2B plays an important role in cellular proliferation and migration. The up-regulation of CSNK2B may be correlated to carcinogenesis of HCC. Silencing of CSNK2B inhibit the cell proliferation and cell migration. The drug tests also confirmed that knocking down CSNK2B increase the cell sensitivity to the antitumor drug. CSNK2B might be developed into a potential target for anti-cancer drug screening.