Epigenetic Regulation Of Expression Of AP1in Kazakh’s Esophageal Cancer Tissues And Cell Lines

Objective:to explore the expressional difference of c-jun, c-fos, MMS22L and Ras51between esophageal squamous cell carcinoma (ESCC) tissues and matched paracarcinoma normal esophageal mucosa, studying the correlation among these genes in ESCC simultaneously; to explore whether histone deacetylase (HDAC) and demethylating agent5-aza involved in the expression of c-jun, c-fos, MMS22L, Rad51and XB130.Methods:1. RT-PCR was sused to detect the expression of c-jun, c-fos, MMS22L and Ras51in40cases of both esophageal squamous cell cancer tissue and matched paracarcinoma normal esophageal mucosa.2. HDAC inhibitor TSA and5-aza was added in the esophageal cell lines ECA109to detect the expressions of c-jun, c-fos, MMS22L and Ras51by RT-PCR.3.with fluorescently labeled pEGFP-N1-Lrigl plasmid transfected in ECA109cell lines overexpressing Lrigl to detect the expressions of c-jun, c-fos, MMS22L and Ras51using RT-PCR and.Results:the expression level of c-fos in cancer tissues was higher than normal tisses, with statical significance (P<0.05); c-jun, Rad51, MMS22L mRNA were slightly higher in cancer tissus than that of normal tissues, but the difference was not statically significant (P>0.05);2.the expression of Rad51in the experimental group induced by TSA and TSA+5-aza comapring with control group was downregulated and c-fos expression in TSA+5-aza was downregulated, the differences were statistically significant; expression of XB130in5-aza and TSA+5-aza was lower than that of conrorl group, with statical significane; there was no difference in c-jun, MMS22L expressions in each experimental group and conrol group;3. Cell transfection results showed that in esophageal cancer cell lines ECA109overexpression of Lrigl may inhibit the expressions of c-fos, Rad51and XB130at the transcriptional level, while the expression of MMS22L compared with the control group, there is an upward trend; Lrigl had no effect on the expression of c-jun. Conclusions:c-fos expression in esophageal cancer was higher than the corresponding normal tissues, indicating that they may participate in Kazakh’s esophageal squamous cell carcinoma incidence and processes;there was correlation between c-jun, MMS22L at the transcriptional leve; there may exsits a possible regualtion in esophageal cancer cell lines EC109berween TSA,5-aza and c-fos, Rad51and XB130genes; In esophageal cancer cell lines ECA109overexpression of Lrigl may suppress the exprssions of c-fos and XB130, there may be some regulatory relationships; expression of Rad51, DNA damage repair gene, was reduced, suggesting Lrigl may inhibit its expression and promote tumor progression; MMS22L was highly expressed in overexpressed Lrigl group, suggesting that overexpression of Lrigl may contribute to the high expression of oncogene MMS22L.