The Research For Treatment Acute Spinal Cord Injury By Intrathecal Injection Methylprednisolone At Different Times

Objective:This study attempts to explore the effect of intrathecal injection MP (methylprednisolone) treatment ASCI (acute spinal cord injury) at different times in rats, to try exploring the damage mechanism of ASCI and providing relevant theoretical data for further research. Methods:Allen’s frame was used to establish SD (Sprague Dawley) rats ASCI models, then treated with MP (experimental group) or normal saline (control group) by intrathecal injection respectively at the0h,3h,6h,8h,12h and24h after injury. Sterile to extract the injured spinal cord tissues and cerebrospinal fluids (CSF) after treatment of24h later, then analyze the differential expression proteins of the injured spinal cord tissues and CSF by iTRAQ (isobaric tags for relative and absolute quantitation and LC-MS/MS (liquid chromatography coupled with tandem mass spectrometry) technology. Results:There are2242proteins which identified in spinal cord tissues of SD rats, the inflammation factor’s expression (Col6a2collagen, Kng111, Prdx2, Fn1Uncharacterized and S100a8) and the neurotrophic factors’ expression (CD9, Apoal, Acsl6, Kif5c, protein14-3-3, Snap25, Pls3, Omg) of MP groups were reduced after MP intrathecal treatment; Meanwhile, there are722proteins which identified in CSF of SD rats, it is interesting that the inflammation factor’s expression (Fnl Uncharacterized and S100a8) and the neurotrophic factors’ expression (protein14-3-3) of MP groups were reduced after MP intrathecal treatment. Conclusion:This experimental study provide the SD rats’ protein expression spectrums of spinal cord tissues and CSF after ASCI by differential proteomics techniques and the MP intrathecal injection therapy is effective, and MP can relieve the secondary inflammatory response after ASCI, but it does not promote the recovery of neural function in a short time.