Doxycycline On Gastric Cancer Cells(BGC-823) Proliferation And Invasion Impacts Research

ObjectiveEffects of doxycycline on gastric cancer cells BGC-823cell proliferation andinvasion explore doxycycline by Notch1signaling pathway in gastric cancer cellproliferation, invasion of possible mechanisms, provide a new basis for the newmeans of gastric cancer, while providing new anticancer drugs for the treatmentof gastric cancer.MethodsRespectively0、5、10、20、40μg/mL final concentration of doxycyclineeffects on human gastric cancer cell line BGC-823, the use of cell proliferationwas measured by MTT assay; affected by flow cytometry cell cycle distribution;to assess cell migration by Transwell chamber; expression Western Blotdetection Notch1, CyclinD1, CyclinE, MMP-9, VEGF, COX-2protein levels.ResultsMTT assay results showed that: the growth of doxycycline BGC-823cellsinhibited at the same time, with the doxycycline concentration, cell inhibition rateincreased; under the same drug concentration, with the role of time increasing,the inhibition rate were gradually increased, indicating that doxycycline inhibitionrate of gastric cancer cells in a time, concentration-dependent, statisticallysignificant difference (Fgroup=102.050, Ftime=617.376, Finteraction=40.552, P<0.05).Flow cytometry showed that: doxycycline role in BGC-823cells after48h, withincreasing concentrations of doxycycline, G0/G1phase ratio gradually increased (F=892.530, P<0.05), while the S phase compared with the control group showsa decreasing trend in the ratio (F=949.002,P<0.05), G2/M phase also decreasedthe proportion (F=139.190,P<0.05). Western blot analysis showed: the role ofdifferent concentrations of doxycycline BGC-823cells after48h, with the effectof doxycycline concentration, increased expression of Notch1, and CyclinD1,CyclinE, MMP-9, COX-2, VEGF expression is gradually reduced, anddose-dependent manner, the existence of these gaps were statisticallysignificant (P<0.05). Using Pearson correlation analysis showed a negativecorrelation Notch-1and MMP-9expression (r=-0.994, P<0.05). Transwellchamber showed: doxycycline intervention BGC-823cells24h hours later, Withthe increase of the concentration of doxycycline effects, the number of cellsthrough Transwell chamber membranes decreases, the gap was statisticallysignificant (P<0.05), and wear filtration membrane cells and drug concentrationswere negatively correlated (r=-0.862, P<0.05).ConclusionsNotch1could inhibit the proliferation and invasion of gastric cancer cellsBGC-823; Doxycycline could inhibit the proliferation and invasion of gastriccancer cells BGC-823; Doxycycline on gastric cancer cells BGC-823inhibitedproliferation and invasion, activation of Notch1signaling pathway is onepossible mechanism.