Mechanism Of Follicular Development Disorders Caused By Testosterone

ObjectivePolycystic ovary syndrome is the most common reason of clinicalreproductive age women follicular developmental disorder disease.Hyperandrogenism is the most typical symptoms of PCOS. In our study,testosterone granulosa cells were cultured in vitro, and to observe itsproliferation, secretion of VEGF, HIF1α, as well as effects on cell microtubulesmicrofilaments. To evaluate the possible causes and mechanisms of folliculardevelopment disorders by the high concentration of serum androgen and toprovide a theoretical basis to the further study of infertility patients in clinicalcontrolled ovarian hyperstimulation with less complications.MethodGranulosa cells were identified and their morphology wasexamined by hematoxylin and eosin (HE) staining, F-actin, andfollicle-stimulating hormone receptor staining. AMH, VEGF, and HIF-1αexpression was examined by quantitative real-time polymerasechain reaction and enzyme-linked immunosorbent assay.ResultTestosterone treatment did not affect granulosa cell morphologybut significantly increased the expression of AMH and VEGF and HIF-1α.Testosterone can regulate the functions of granulosa cells byup-regulating AMHmRNA and VEGFmRNA ConclusionTestosterone can promote granulosa cell culture medium AMH,VEGF,HIF1ɑsecretion levels, while cells increased significantlyAMHmRNA,VEGFmRNA, HIF1ɑmRNA no significant changes.Testosterone has no significant effect on actin cytoskeleton (F-actin) synthesisdecomposition, and does not affect the normal form of the nucleus.