| Objective:To explore the inhibition effect of the pigment epithelium-derived factor (PEDF) on blood vessel by observing retinal vascular changes of Retinopathy of premature children (ROP) mouse model.Methods:1707-day-old Wister rats were divided into ROP group, normal control group and the treatment group. Compared with neonatal rats’retinal vascular from normal control group, the changes of hyperoxic affected on retinal vascular morphology were observed. The left eyes of the mice in the treatment group were defined as a control, the right eyes of ROP mice were injected with PEDF in accordance with the different doses and injection times. Retinal vascular morphology observations were done and the cell numbers of blood vessel endothelium were counted at17days.Results:The nuclear numbers of left and right eyes in1μg groups were (39.18±3.25) and (14.01±2.04), the nuclear numbers of left and right eyes in2μg groups were (48.41±6.03) and (11.37±1.41). Vascular morphology of the two groups was significantly changed. Number of vascular endothelial cells of the right eyes compared with the left eyes was significantly reduced (1μg group:t=6.53, P=0.001;2μg group:t=5.403, P=0.001).2μg as PEDF dose, one time injection was invalid, twice and three times were effective.Conclusion:PEDF can inhibit the newborn rats’retinopathy model which was induced by hyperoxia. |
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