The Expression And Biological Function Of Mov10in Hepatocellular Carcinoma

HCC is the fifth most common cancer worldwide and the third mostcommon cause of cancer mortality. As the most frequently occurringprimary hepatic malignancy, HCC accounts for between85%and90%.HCC has several epidemiologic features including dynamic temporal trends;marked variations among geographic regions, racial and ethnic groups, andbetween men and women; and the presence of several well-documentedenvironmental potentially preventable risk factors. The dismal prognosis ofadvanced HCC mainly due to patients diagnosed late, the high recurrencerate and the limit effect of chemotherapy. These unfavorable survivalstatistics clearly define a need for improved medical treatments anddiagnosis.Mov10protein is a member of RNA helicase-1superfamily, and it isalso one of the components of the RNA-induced silencing complex. Recentstudies have shown that Mov10plays an important role inmiRNA/siRNA-induced gene silencing and potently inhibits HIV-1replication at multiple stages, including virus production, proteolytic processing of Gag, and reverse transcription. Meanwhile, Mov10canreduce the infectivity of other retrovirus such as simian immunodeficiencyvirus, murine leukemia virus and equine infectious anemia virus. The studyby Nakano et al firstly described that Mov10overexpressed in tumors andspeculated to involve carcinogenesis and differentiation relating to hTERTand telomere.In accordance with the research by Nakano et al, our study indicatethat the expression level of Mov10in hepatocellular carcinoma wassignificantly higher than that in healthy liver. The same tendency was foundin hepatoma cell lines compared with normal liver cell lines. In this study,HepG2cell line stably overexpressing Mov10protein was established andthe role of Mov10in cell proliferation and invasion was studiedpreliminarily. This stable cell line was verified to exogenous expressMov10by using of real-time PCR, western blotting andimmunofluorescence. Mov10is likely to accelerated proliferation andinvasion of tumor cells, and may enhance colony-formation viability.