The Expression Of TGF-β1and α-SMA Betweenb Young And Adult Ratsa After Intracortical Injection Of Ferric Chloride

Objective: To explore the correlation between the expressions ofTGF-β1&α-SMA and seizures of rats after intracortical injection of ferricchloride and explore its role in occurrence of rats seizure.Methods: Rats(childhood and adulthood)were treated with anintracortical injection of FeCl3and normal saline，the occurrence of seizureswere under observation;TGF-β1in cerebral spinal fluid（CSF）was tested byELISA;the expressions of mRNA and protein of α-SMA were detected byRT-PCR and Western blotting.Results: Epilepsy seizure was only observed in FeCl3childhood group；TGF-β1and α-SMA expression ranks top1in FeCl3childhood group.Conclusion: Childhood rats were more susceptible to seizure thanadulthood rats with higher level of TGF-β1and α-SMA expressions；implying that the growth of TGF-β1and α-SMA expressions might be theone of key mechanisms in seizure.