Neuroprotection And The Mechanism Of Short-term Sleep Deprivation On Hippocampal Neuron Injury In Global Cerebral Ischemia Reperfusion Rat

Objective: To investigate the effect and the mechanism of short-termsleep deprivation on the hippocampal neuron injury in global cerebralischemia reperfusion rat．Methods:Rat model of global cerebral ischemia reperfusion(GCIR) wasestablished by bilateral common carotid artery occlusion for20minuteswith bloodletting from the Jugular vein followed by reperfusion for48h.Rats were randomly divided into5groups: Sham operation group;GCIR treated group，the rats were subjected to treatment of GCIR;GCIR+6hSD*3d treated group，rats were subjected to treatment of GCIRfollowed by sleep deprivation treatment for6h/d，continuously3d；GCIR+12hSD treated group，rats were subjected to treatment of GCIR followedby sleep deprivation treatment for12h; GCIR+12hSD*3d treated group，rats were subjected to treatment of GCIR followed by sleep deprivationtreatment for12h/d，continuously3d.Morris water maze test was used to detect learning and memoryfunction of rat. Pathophysiological changes of rat hippocampal neuron was observed by the method of HE staining. TUNEL staining was used to detectthe apoptosis of rat hippocampal neurons. The methods of BrdU and PCNAImmunohistochemistry were carried out to detect the neuron proliferation.BrdU/NSE Immunofluorescence technique was used to detect celldifferentiation. The method of ELISA was used to measure the contents ofBDNF in the rat hippocampus.Results: Short-term sleep deprivation significantly improvedimpairment of learning and memory function in global cerebral ischemiareperfusion rats. Short-term sleep deprivation also significantly preventedhippocampal neuron from karyopyknosis, reduced number of apoptoticneurons in the rat hippocampus CA1area, enhancing the proliferation anddifferentiation of neural stem cell and increased the content of BDNF inglobal cerebral ischemia reperfusion hippocampus in rats.Conclusions: Short-term sleep deprivation can improve brain neuroninjury and impairment of learning and memory function in global cerebralischemia reperfusion rat. The neuroprotective mechalism may result fromincreasing the content of BDNF, preventing neuron apoptosis of rathippocampus and promoting the proliferation and differentiation of neuralstem cells in global cerebral ischemia reperfusion rats.