The Biological Effect And Mechanism Of HnRNPA2B1Gene Silencing On786-0Renal Cell Carcinoma Cells

Objective: To investigate the biological role of hnRNPA2B1gene onthe growth of786-0cells and its potential mechanism.Method：HnRNPA2B1shRNA recombinant plasmids were constructedand transfected into renal cell carcinoma786-0cells. The monoclonal celllines which transfected by plasmid (including the empty plasmid) wereselected by G418. The effects of hnRNPA2B1shRNA recombinant plasmidson hnRNPA2B1gene silencing were detected by qRT-PCR. The models ofhnRNPA2B1gene specific lower expression were established in786-0cells.Cells were divided into3groups according to differentinterventions:P-shRNA-A2B1group (transfected by plasmid),P-shRNA-GFP group (transfected with empty plasmid), control group(786-0cells without any treatment). MTT method was used to observe theproliferation ability of786-0cells in different groups.The apoptosis of786-0cells were assayed by Flow cytometry.The mRNA expression of Bcl-X(S)and cl-X(L)were detected by qRT-PCR.The ratio between Bcl-X（S）andBcl-X（L）was calculated.Result:1、The hnRNPA2B1gene expression level in P-shRNA-A2B1 group was significantly lower than the control group(P <0.05),while therewas no significant difference between the P-shRNA-GFP and the controlgroup(P>0.05). That is to say the model of hnRNPA2B1gene lowerexpression in786-0cells have been successfully established;2、Compared tocontrol groups, the apoptosis rates of P-shRNA-A2B1groups wereincreased significantly while the cell multiplication rates were notablydecreased（P<0.05）,but there is no different between the P-shRNA-GFP andthe control group(P>0.05);3、Compared with the control groups,the mRNAexpression levels of Bcl-X（S）and the ratio of Bcl-X（S）/Bcl-X（L）in P-shRNA-A2B1groups were increased significantly（P<0.05），but thereis no different between the P-shRNA-GFP and the control groups (P>0.05).Conclusion: hnRNPA2B1were express in786-0cells.hnRNPA2B1gene silencing could effectively inhibits the proliferation of786-0cellswhile apoptosis is promoted, the mechanism of which probably relates to itsaffection on the expression of Bcl-X（S）and Bcl-X（L）.