The Influence Of SAA Overexpression On Nasopharyngeal Carcinoma CNE2Cell Proliferation

ObjectiveTo construct an eukaryotic expression vector pcDNA3.1(+)-SAA and therecombinant plasmid pcDNA3.1(+)-SAA transfected into CNE2cell,To detect itsexpression in CNE2cell and the influences of its expression on CNE2cellproliferation by MTT.MethodsHuman SAA gene was amplified by PCR with CNE2’s cDNA as the templateand the fragment was combined with plasmid pcDNA3.1(+).The recombinantexpression vector, pcDNA3.1(+)-SAA was constructed successfully afteridentification by sequencing appraisa and Blast analysisl.The recombinant expressionvector, pcDNA3.1(+)-SAA was transfected to CNE2cell usingLipofectamineTM2000. The expression level of SAA gene before and aftertransfection was detected by Western blotting.The influences of its expression onCNE2cell proliferation was futher analyzed through MTT.Results1、 After identification by restriction enzyme digestion and sequencing, theeukaryotic expression vector, pcDNA3.1(+)-SAA was successfully constructed.2、The Western blot results show that the expression level of SAA in CNE2celltransfected by pcDNA3.1(+)-SAA was significantly higher than that in the control（P＜0.05）. 3、it was found from MTT testing result that after transfect the recombinantplasmid CNE2proliferation was faster than the control（P＜0.05）.Conclusion1、pcDNA3.1(+)-SAA can express in CNE2;2、Serum Amyloid A(SAA) could bloost CNE2proliferation.