Analysis The Viability And The Transplanted Survival Rate In Cryopreservation Of Fat Granule With Different Frozen Conditions

Background Autologous fat tissue is an ideal filler material to the defects of softtissue, it almost can be used to fill soft tissue defect in various areas of the body.Although in order to improve the survival rate and reduce the absorption rate, theresearchers conducted a great deal of research at home and abroad, but in the endthe transplantation of adipose tissue volume can only keep in transplantation ofaround50%. Clinically, therefore, repeatedly transplant even by a small amounteach time is needed to achieve a satisfactory filling effect. Fat injections for manytimes increased the workload of doctors, and liposuction pain, high risks and costsof patients. It shows important clinical application value for us to achieve a drawenough fat in vitro frozen storage for a long time, and injection after thawingcultivate when necessary. Current research on particle adipose tissue protectiveagent at low temperature choice is limited. Research of whether the commonlyused basic medium of cryopreserved cells can help to maintain the vitality ofcryopreserved adipose tissue, enhance the survival rate of transplant is stillwithout report.Objective To investigate whether the high glucose Dulbecco’s Modified EagleMedium and fetal bovine serum as base fluid are benefit for improve the viability andpost-transplant survival rate of frozen adipose tissue.Methods Fat granules were cryopreserved at-196℃in physiological saline, highglucose Dulbecco’s Modified Eagle Medium and fetal bovine serum respectively,adding with8%DMSO. Rewarming after2months, the viability and posttransplantation survival rate of frozen fat were determined by trypan blue staining, creatine kinase activity assay, MTT assay.Results The viability and post transplantation survival rate of fat granule frozenin physiological saline and high glucose Dulbecco’s Modified Eagle Medium,adding with8%DMSO, were displayed no difference. However, frozen adiposetissue in fetal bovine serum adding with8%DMSO showed an increase in cellviability and improvement in post transplantation survival rate from52.19±2.59%to73.91±1.20%.Conclusion FBS as a base fluid can significantly improve the cell vitality and posttransplantation survival rate of cryopreservation adipose tissue.