Analysis The Activity Of Fat Granule With Different Frozen Conditions

Background:people fat particles because of its rich source and no scar, high survival rate,complementarity-mutual exclusiveness, and many other advantages, has becomethe preffered filler for soft tissue defect of the various areas of the body. With thevigorous development of cosmetic industry in our country in recent years,autologous fat granules transplantation has been more and more widely used inclinical treatment. But because of the transplant survival rate is not high, can onlystay in transplantation of around50%, the transplantation was needed to be donerepeatedly to achieve satisfactory results. Repeatedly fat transplantation can causepatients suffering discomfort, but also increased the operation risk and economicburden of patients and increase the workload of doctors. If we can find a way oflong-term preservation of fat, to achieve liposuction one time but used multipletimes, have important clinical practical value. At present the main means of fatpreservation is kept at low temperature, but at what temperature,the activity ofhuman fat is strongest, which is the best way to save the fat, there has been norelevant report.ObjectiveObserve the human fat in different freezing conditions by HE dyeing.thepercentage of fat cells by blue dyeing under inverted microscope tyres and detectthe ability of cell,s proliferation by MTT method.In order to find the best way topreserve fat in long time and provide the theory basis for its clinical use.MethodsThe human fat tissue was divided into three groups:-20℃(ordinaryrefrigerator frozen)group,-80℃(deep cryogenic refrigerator)group,-196℃(liquid nitrogen)group, and then took out the human fat tissue respectively after:2weeks,8weeks and24weeks. Observe the human fat in different freezing conditions by HE dyeing and Oil red O staining,the percentage of fat cells by bluedyeing under inverted microscope tyres and detect the ability of cell,s proliferationby MTT method Reslut:It was not obvious in the cell,s survival rate and activitywhen fat cells were perserved one week respectively at-20℃、-80℃and-196℃. The fat cell,s survival rate and activity of-20℃group was significantlylower than-80℃and-196℃group after12weeks and24weeks. But,it was notobvious in the cell,s survival rate and activity when fat cells were perserved1week、12weeks and24weeks respectively at-80℃a nd-196℃.Results:1．Adipose tissue preserved under-20℃after1week in cell survival andcell activity compared with-80℃groupand-196℃group is not very different.-20℃stored for12weeks,24weeks after the adipose tissue cell survival rate andcell activity was significantly lower than-80℃and-196℃group (p<0.05).2．Under-80℃and-196℃stored for1week,12weeks,24weeksrespectly,the cell survival rate and cell activity of the adipose tissue had no significantdifference.Conclusion：1．Fat tissue is not suitable for long-term preservation at-20℃(ordinaryrefrigerator lattice) and is suitable for short-term storage.2．.-80℃(deep freezer),-196℃(liquid nitrogen) groupare suitable forlong-term preservation and they could meet the demand of clinicaltransplantation.