The Expression And Relevance Of Tbx3and PTEN In Primary Spinal Cord Gliomas

Background&Objective Primary intramedullary spinal cord tumor accounts forabout20%of the tumor in the spinal canal. however, primary spinal cord gliomas canbe occupied to intramedullary spinal cord tumor above80%. The major type of spinalcord gliomas includes Ependymoma, Astrocytoma and Oligodendroglioma. At present,the major therapy of spinal cord gliomas is still surgical operation with radiotherapyand chemotherapy after operation. However, the prognosis of this disease is poor,because of high risk of recurrence and nerve function complications. As the developof molecular biology. Target treatment and genetic therapy may provide new ways tocure this disease. However, the research about intramedullary spinal cord tumor is notvery deep.PTEN is another important cancer repressing gene after p53. the protein itexpresses is a kind of factor which can inhibit tumors. It can prohibit over-speeding ofcell proliferation and repress the uncontrolled cell splitting, so that it can regulate celldivision cycle. Tbx3is a member of T-box gene family. It expresses the protein whichcan stimulate the proliferation of embryonic stem cell. Recent researches have foundthat it accounts for many kinds of tumor genesis, especially for breast cancer.Researches have found that Tbx3can repress the expression of PTEN.The research uses immunohistochemical staining technique to retrospectiveanalysis the expression of Tbx3and PTEN in primary spinal cord gliomas and itsrelationship between two factors, So that we can discuss the mechanism of spinal cordgliomas tumorgenesis from molecular biology.Method We collected52cases which are make a definite diagnosis of primary spinalcord gliomas from2010to2013. these cases include30ependymomas,12astrocytomas and10oligodendrogliomas. They are divided into3groups by theirpathological pattern.10cases of normal spinal cord take as control group. useingimmunohistochemical staining technique to analysis the expression of Tbx3and PTEN in primary spinal cord gliomas. The result of staining has been recorded byimage capture system, then describing the result by MOD. The date is analysised bySPSS19.0statistical software, using one-way ANOVA and Pearson CorrelationAnalysis.Result the MOD of Tbx3in experimental groups are obviously higher than controlgroup(p<0.05). in each experimental group, it has no obvious difference betweenependymoma group and astrocytoma group(p>0.05), however, astrocytoma group hasobvious difference with ependymoma group and oligodendroglioma group(p<0.05),its MOD is lower than ependymoma group and oligodendroglioma group.On the other hand, the MOD of PTEN in experimental groups are obviouslylower than control group(p<0.05). in each experimental group, there is no obviousdifference between ependymoma group and astrocytoma group(p>0.05), but,astrocytoma group has obvious difference with ependymoma group andoligodendroglioma group(p<0.05), its MOD of PTEN is higher than ependymomagroup and oligodendroglioma group.In the experimental groups and control group, it has statistical significancenegative correlation between the expression of Tbx3and PTEN.Conclusions1.the Tbx3expresses in most of primary spinal cord gliomas, but almostno expression in normal spinal cord. However, the intensity of expression inastrocytoma is lower than oligodendroglioma.2.the intensity of PTEN expression inmost of primary spinal cord gliomas is lower than normal spinal cord. but, theintensity of expression in astrocytoma is little higher than oligodendroglioma.3.inprimary spinal cord gliomas, Tbx3is over-expressed, on the contrary, PTEN is low-expressed. It has negative correlation between the expression of Tbx3and PTEN.