The Effect Of Ulinastatin And Docetaxel On Breast Cancer Immuno-microenviroment

Objective:To investigate the effect of ulinastatin(UTI) anddocetaxel(DCT) on immunocyte, immune factors and matrixes in breastcancer immuno-microenvironment, the tumor growth and metastasis.Methods:4T1breast cancer cell culture,the purchase BALB/c mice,theestablishment of BALB/c mice bearing4T1cells model.32mice wererandomly divided into four groups:Control group, UTI group,DCT groupand UTI+DCT group.Measure tumor size and calculate inhibitionrate.Line flow cytometric dyeing,detection CD4+CD25+Foxp3+Tregcontent.The expression of interleukin-2(IL-2),interleukin-6（IL-6） andinterleukin-10（IL-10） was detected by ELISA.The expression of matrixmetalloproteinases-9(MMP-9) and tissue inhibitor ofmetalloproteinase1(TIMP1) was detected by Western Blot.Metastasis wasdetected by HE staining.Results:Compared with the control group, both the UTI and DCTinhibit tumor growth,and when UTI combined DCT,the inhibition was themost significant(P=0.000).After the treatment of UTI,DCT and both ofthem,compared with the control group,the rate of CD4+CD25+Foxp3+Treg/CD4+T cells was decreased(P=0.000), theexpression of IL-6,IL-10and MMP-9was significant lower(P=0.000),while promoting the expression of IL-2and TIMP1(P=0.000).UTIcombined DCT significantly inhibited tumor metastasis.Conclusion:UTI combined DCT suppress the tumor growth andmetastasis in immune normal mice.The mechanism may be related toregulating CD4+CD25+Foxp3+Treg, IL-2, IL-6, IL-10, MMP-9and TIMP1in the tumor microenvironment.