A Preliminary Study Of The Injury And Its Clinical Significance In Patients With Chronic Prostatitis Sperm DNA

Objectives: Explore the different causes of chronic prostatitis patients a merger ofsperm DNA damage, DNA damage in sperm learn more about the different causes ofchronic prostatitis caused by the presence or absence of different.Methods: Through the160cases of prostatic fluid into the case by the results ofroutine laboratory screening of chronic prostatitis, parallel semen analysis, theirprostatic fluid test results will be divided into groups of chronic prostatitis, bacterialinfection (A group of40patients), chronic prostatitis mycoplasma infection (B groupof40patients), combined with other causes of chronic prostatitis group (C group of40patients) and the healthy control group (D group of40patients). Then the spermchromatin were used diffusion test and comet assay two kinds of methods were usedto detect patients with sperm DNA integrity, in which the sperm chromatin diffusionexperiments with sperm DNA fragmentation index indicates the extent of damage,namely the qualitative test sperm DNA damage, comet assay percentage of tail DNAcontent expressed their degree of injury, the result of sperm chromatin diffusionexperiments were further verified that sperm DNA damage quantification experiments.Finally, statistical analysis of A, B, C, D four groups are merged between the degreeof sperm DNA damage and set group to learn more about different causes of spermDNA damage caused by the presence of chronic prostatitis different.Results:Line160cases of sperm chromatin diffusion experiments drawn: bacterialinfection, mycoplasma infection, other causes SCD group was higher than thehealthy control group SCD injury rate, the difference was statistically significant(p<0.005). SCD damage bacterial infection group was higher than the group ofmycoplasma infection, injury rates for other reasons SCD group, the difference wasstatistically significant (p<0.005). Mycoplasma infection and other causes of SCDinjury rate SCD injury rate between groups was not statistically significant (p>0.05).Comet assay results: Bacterial infection comet tail length as a percentage of47.44±25.80，mycoplasma infection comet tail length as a percentage of22.96±21.40, forother reasons set percentage of comet tail length of22.02±21.92, healthy controlgroup comet tail length as a percentage of6.51±9.85. Bacterial infection，mycoplasma infection, other causes group comet tail length percentage higher thanthe percentage of healthy control group comet tail length, and the difference wasstatistically significant (p<0.005). Bacterial infection comet tail length greater than thepercentage of mycoplasma infection and other reasons set percentage of comet taillength, the difference was statistically significant (p<0.005), mycoplasma infectioncomet tail length and other reasons set percentage of comet tail length was no statistical percentage significance (p>0.05). Can be considered: between sperm DNAdamage in each experimental group and control group differences, sperm DNAdamage in each experimental group was higher than the healthy control group. SpermDNA damage in group A than in group B, sperm DNA damage group C. But still cannot believe that there is a difference in group B and group rates of sperm DNAdamage C.Conclusion:1. Chronic prostatitis can cause varying degrees of sperm DNA damage.2.Different causes of chronic prostatitis, different sperm DNA damage, with thedegree of bacterial infections caused by DNA damage sperm worst, mycoplasmainfection and other causes of chronic prostatitis sperm DNA may also have differentdegrees of damage.3. Detection of sperm DNA damage may be the study of male infertility clinic is animportant indicator.