| Objective: By exploring the distribution frequency and characteristics ofthree common single-nucleotide polymorphisms (SNPs) of NAD(P)H: quinineoxidoreductase1(NQOl) gene promoter of people in GuangXi Guilin area, toanalysis the relationship between NQOl polymorphisms and breast cancer inGuangxi Guilin area plays an important role in this study. In addition, the studyalso approaches the association between genotypes and menstruation, engenderand hereditary factors as well as the interaction between genetic andenvironmental factors occurred in breast cancer. Furthermore, we also quest thecorrelativity between estrogen receptor and NQO1polymorphisms. All aboveexperiments are in order to esploring if NQOl polymorphisms associated withthe risk of breast cancer, and seeking the molecular markers of breast cancersusceptibilities further.Methods: A case-control study based on hospital was carried out and allthe objects were frequency matched by259breast cancer cases-298normalcontrol. All the subjects were recruited from the Affiliated Hospital of GuilinMedical University during March,2011to July,2010. All the subjects weresurveyed face to face by the specifically trained investigators, including basicdatum and environment exposure factors. Clinic data were add by medicalrecords. TaqMan MGB Real-Time fluorescence quantitative PCR technologybased on ABI platform was applied to detect the SNPs of NQO1C609T (rs1800566). All data were input and analyzed statistically in SPSS19.0forWindows. The interaction of gene-environment was analyzed by Logisticregression model and assessed by the synergy index S as well as theHardy-Weinberg equilibrium were analyzed in HaploView4.2.Results:(1) A total of557subjects with complete information wererecruited,259for breast cancer patients and298for normal controls. There wereno statistically differences in the distributions of age and nation, but significantstatistically difference in environment factors such as BMI, menarche age lessthan or equal to12years old, irregular menstruation, the age of firstdelivery of abortion is greater than or equal to28years old, nanparous, nobreast-feed, abortion, family history of breast cancer and personal history ofbenign breast disease (P<0.05).(2) There was obvious significant statisticallydifference in the polymorphisms of NQO1among the cases and controls (P＜0.05).(3)he interaction of menarche age less than or equal to12years old,the age of first delivery of abortion is greater than or equal to28years old,abortion, family history of breast cancer and personal history of benign breastdisease are the important risks of breast cancer (P<0.05).(4) The estrogenreceptor positive rate increased while the breast cancer patients carries1~2mutations.Conclusions:(1) There was obvious significant statistically difference inthe polymorphisms of NQO1among breast cancer patients and normal controls.(2) The interaction of menarche age less than or equal to12years old,the age of first delivery of abortion is greater than or equal to28years old,abortion, family history of breast cancer and personal history of benign breastdisease are the important risks of breast cancer. NQO1gene C609T (rs1800566)polymorphism and the presence of the interaction of these factors, the incidence of breast cancer may be synergistic. There was synergistic association betweenNQO1polymorphisms and the factors mentioned above to enhancing the risk ofbreast cancer, they may synergistically promote the attack of breast cancer.(3)Compared with wild type, the positive rate of estrogen receptor in breastcancer patients with1~2mutant genes is much higher and HER-2receptorcontrast. It maybe due to activity weakened of the enzyme while NQO1genepolymorphism occurs, can not effectively antagonize oxidative damage causedto estrogen and progesterone in breast tissue. |
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