Expression Of Annexin A2and C-myc In Esophageal Squamous Cell Carcinoma And Relationship Between It And Proliferation,Apoptosis

Background and Purpose:Esophageal squamous cell carcinoma(ESCC) is a common digestive tract cancer with a relatively higher mortality rate, and now it has become a very prominent public health problem, its ineidence and mortality rate in China is the highest than the world’s,the mortality rate ranks second among the digestive tract tumors,Esophageal squamous carcinoma and other human cancer similar,its occurrence and environmental factors and related genome instability relevant, belong to many factors and many steps generating process, and the original the activation of oncogene and tumor suppressor gene inactivation relevant.Annexin A2protein is an important member of the calcium phospholipids bindingprotein family (Annexins). It’s encoding gene is located on chromosome15q21-q22. Annexin A2protein plays an important role in a series of calcium-mediated cell activities such as endocytosis and exocytosis, biofilm accumulation, ion channel formation,cell proliferation and apoptosis,etc. In recent years,the over- expression of Annexin A2has been observed in breast cancer, colon cancer,lung cancer,and primary renal cell carcinoma.But another study showed that the depressing expression of Annexin A2protein was observed in prostate cancer and esophageal squamous cell carcinoma. Therefore, the relationship between Annexin A2protein and tumor needs for further study.c-myc is a more conservative evolution of transcription regulatory factor, it participate in the regulation of cell proliferation,differentiation,growth, and malignant transformation of cells. Researches show that Annexin A2can bind with the mRNA of c-myc and prolong its half-life so that increase the expression of c-myc.The research about combined detection of Annexin A2,c-myc expression in ESCC and its relationship with tumor has not been reported. The objective of the study was in an attempt to identify the expression pattern of c-myc and Annexin A2in ESCC and adjacent normal mucosa tissues by in situ hybridization and immunohistochemical staining techniques. Also, we analyed their correlations with clinicopathological features, then discussed their roles in the occurrence, development,invasion,metastasis and prognosis of ESCC.It analyzes cell proliferation and apoptosis state in tissue of the the esophageal squamous cell carcinoma with TUNEL method and Ki-67expression. Explore the relationship of Annexin A2,c-myc and esophageal cancer cell apoptosis simultaneouslyMaterials and methods:1.Using immunohistochemical SP method to detect60cases of esophageal squamous carcinoma tissue,45cases of carcinoma side not typical hyperplasia group and60cases of normal esophageal mucosa tissue of the Annexin A2,c-myc protein expression.2. Using the in situ hybridization technical detection60cases of esophageal squamous carcinoma tissue,45cases of carcinoma side not typical hyperplasia group and60cases of normal esophageal mucosa tissue of the Annexin A2,c-myc mRNA expression.3.Using the TUNEL method analyzed apoptosis state in tissue of the the esophageal squamous cell carcinoma. 4.The statistical treatment:use SPSS17.0software to carry on statistical analysis,inspection level for α=0.05.Results:1.The positive rates of Annexin A2protein(65%)and mRNA(71.67%)in ESCC were significantly lower than in adjacent atypical hyperplasia (77.78%,86.67%)and in normal esophageal mucosa(96.67%,98.33%).There were significant differences in the expressions of c-myc among the three groups(p<0.05). The strength of Annexin A2protein and mRNA expression in ESCC didn’t associated with,age gender,and size of tumor(P>0.05),but with the depth of degree of differentiation,TNM staging and lymph node metastasis(p<0.05).2.The positive rates of c-myc protein(61.67%)and mRNA(68.33%)in ESCC were significantly higher than in adjacent atypical hyperplasia (46.67%,53.55%)and in normal esophageal mucosa(31.67%,15%).There were significant differences in the expressions of c-myc among the three groups(p<0.05). The strength of Annexin A2protein and mRNA expression in ESCC didn’t associated with,age gender,and size of tumor(P>0.05),but with the depth of degree of differentiation,TNM staging, and lymph node metastasis(p<0.05).3.The expression of Annexin A2and c-myc in ESCC showed a positive correlation through the statistical analysis (P<0.05).4. Tumor cell apoptosis in ESCC has relationship with Annexin A2protein and mRNA expression. Tumor cells AI in telomerase-positive expression group was (4.39±2.03)%and (6.35±2.33)%, it is lower than the negative expression group (11.46±3.62)%and (11.91±3.39)%,the difference was statistical significant (P<0.01).5. The expression of Annexin A2and Ki-67in ESCC showed a positive correlation through the statistical analysis (P<0.05).Conclusion:1. Low expression of Annexin A2and high expression of c-myc are closely related to the occurrence and development of ESCC. Annexin A2and c-myc may have synergy in the process of occurrence and development of ESCC. 2. Low expression of Annexin A2and high expression of c-myc in ESCC are significantly correlated to degree of differentiation,lymph node metastasis and TNM stage of tumor; it is indicated that the abnormal expression of Annexin A2and c-myc are closely correlated to the invasion, metastasis and prognosis of ESCC.3. Annexin A2involved in apoptosis and proliferation of esophageal squamous cell carcinoma.