Effect On Transplantation Of Bone Marrow Mononuclear Cells On Neural Restoration In Rats With Intracerebral Hemorrhage

BackgroundCerebral hemorrhage with high mortality and high morbidity characteristics is acommon clinical cerebrovascular disease, cerebral hemorrhage hemorrhage leading tothe central area of the nerve cell death neurological deficit, has truly lacking effectivetreatment and drugs, currently transplantation has the ability to self-renewal anddirected differentiation of exogenous stem cells are pluripotent treatment of cerebralhemorrhage most potent, causing bone marrow mononuclear cells extracted easilywith no need medium to autograft and many other advantages, making the treatmentof acute one of the best seed cells of cerebral hemorrhage, vein graft BMMNCs latestresearch proves safe and effective, and can be directed to differentiate into a varietyof cells in the induction of a specific microenvironment.ObjectiveTo investigate the effect of transplantation of bone marrow mononuclear cells(BMMNCs) on neuron-like cells and astrocyte-like cells differentiation, expression ofglial cell-derived neurotrophic factor (GDNF) in rats with intracerebral hemorrhage.MethodsIntracerebral hemorrhage (ICH) was induced by stereotactic injection of type Ⅳcollagenase into the striatum of rats, the successful models were randomly dividedinto the model control group (ICH group) and BMMNCs transplantation group(BMMNCs group), BMMNCs groups was injected1mL cell suspension containing3×107groups. The neurological functions of the rats were observed by modified neurological severity scores (mNSS) test on days1,7,14and28after the injection;The expression of GDNF in cerebral tissue was observed by Western blotting on days1,7,14after the injection; Double immunofluorescent labeling was utilized to tracethe differentiation of BMMNCs in the rats’ brains on days28after the injection.ResultsAt7th days、14th days、28th days, the mNSS scores in the BMMNCs group weresignificantly lower than the ICH group, difference was statistically significant(P<0.05); The GDNF expression in both groups began to appear1day after ICH,peaked on day7and gradually reduced on day14; at each observation time, theGDNF expression in ICH group was significantly lower than BMMNCs group,difference was statistically significant (P<0.05); At28th days, Brdu and neuronspecific nuclear protein (NeuN) double-labeled positive cells and Brdu and glialfibrillary acidic protein (GFAP) double-labeled positive cells were found inhemorrhage in the BMMNCs group.ConclusionBMMNCs transplanted can improve the neural behavioral function, increase theexpression of GDNF, and differentiate into neuron-like cells and astrocyte-like cells.