The Effect Of Esomeprazole On Human AGS Gastric Cancer Cells

ObjectiveThe purpose of this report is to detect the effect of esomeprazole on proliferation,migration, invasion, apoptosis and cycle of human gastric cancer AGS cell, inaddition, to provide the basic experimental evidence which supports the esomeprazoleas a selective antineoplastic scheme.MethodsApply MTT colorimetry to analyse the effect of esomeprazole or combinedchemotherapy drugs on gastric cancer cells proliferation.Construct transwell model toobserve the effect of esomeprazole or combined chemotherapy drugs on migrationand invasion of tumor cells.Implement flow cytometry to discover the changes ofapoptosis and cycle of drugs measured gastric cancer cells.ResultThrough the MTT colorimetry, we find that after48hours of treatment on cells,while the concentration of esomeprazole is greater than10μg/ml, the effect on theproliferation of tumor cells increases as the increment of concentration; compared with control group. this outcome has statistical significance (P<0.05). Treating cellswith different concentration of esomeprazole (10,20,40μg/ml) and combined withADM, DDP, or ADM plus DDP at24,48, or72hour, the inhibition rate of AGS cellspresents a time-dose dependent effect.By constructing a transwell model, we discover that after12hours of treatmentby esomeprazole on gastric cells, the amount of cells which pass through membranedecreases as the concentration of esomeprazole increases. Compared with controlgroup (P<0.05), the quantity of cells which passed through membrane minimisedwhile treating cells with esomeprazole combined ADM plus DDP.Double staining flow cytometry has been implemented onto the effect ofesomeprazole combined ADM+DDP on AGS cells; as a result, esomeprazole mayincreases the proportion of early apoptosis and necrotic cells in AGS cells. The scaleof early apoptosis and necrotic cells in gastric cells is proportional to theconcentration of esomeprazole. The comparison between control group (only usechemotherapy drugs) and experimental group (use esomeprazole and ADM+DDP) atthe proportion of early apoptosis and necrotic cells has statistical significance(P<0.05).Propidium Iodide flow cytometry is used to evaluate the effect of esomeprazole(10μg/ml) on AGS cells cycle after48hours treatment; the amount of AGS cells atG1/G0phase increased gradually while cells at S phase and G2/M phase decreased, ithas statistical significance while compared with control group (P<0.05). The amountof AGS cells at G1/G0phase decreased vastly if implemented with esomeprazolecombined ADM+DDP, while the cells at S phase increased significantly and cells atG2/M phase slightly increased. This outcome is different with the control groupwhich only implementing chemotherapy drugs (P<0.05).Conclusion(1)Apply esomeprazole independently can inhibit gastric cancer cellsproliferation, migration and invasion of tumor cells, and also induce tumor cellsapoptosis. (2)Esomeprazole can disrupt cell cycle; independently applying esomeprazolewould block the G1phase to S phase of cells, which results in the amount of cells atG1increases, but the proportion of cells at S phase and G2phase decrease. Thecombination of esomeprazole and chemotherapy drugs would block thetransformation of cells at S phase. which cause a great amount of cells at S phaseaccumulate, hence disrupt the AGS cell cycle effectively. In conclusion,esomeprazole is able to improve the antitumor effect of chemotherapy drugs.