| Sea cucumber, which belongs to class of Holothurioidea from phylum ofEchinodermate, has high nutritional value and medicinal value. It was recorded inCompendium of Materia Medica since the Ming Dynasty and classified as tonics.According to the records, sea cucumber has many benefits of protecting kidney andblood vessel. Cancer is one of the main threatening diseases to human health. Mostchemotherapy drugs would cause damage to the bone marrow on different degrees,which lead to a reduction of hematopoietic function and anemia of patients.Glycosaminoglycan in present study (HGAG) is extracted from Sea CucumberApostichopus Japonius, which possess the activities of antitumor, immune regulation,anticoagulation, and anti-aging activity. However, the reports on its hematopoieticfunction are rare. The present study investigated the effect of HGAG onhaematogensis function in myelosuppressed anemia mice through the administrationin vivo and in vitro. The prior study has great benefit on the further biologicalresearch of sea cucumber polysaccharide and the development of new antitumor drug.The main conclusions of the research were shown as follows:To observe the effect of HGAG on haematogensis function in anemia mice bythe administration in vivo.40male kunming mice were randomly divided into normalgroup, model group, three different experiment groups (dosage of administration:1,5,15mg·kg1, respectively). Except the normal group, the rest four groups were made tothe anemic model by injection of cyclophosphamide (100mg·kg1) once a day forthree days. At day one, three experiment groups were intraperitoneal injection withHGAG, and normal group and model group were injected with saline solution, once aday for seven days. The effects of HGAG on peripheral blood cells, bone marrownucleated cells (BMNCs), spleen indices and bone marrow cell cycle in anemia micewere detected on day7. The result showed that peripheral white blood cells andhemoglobin were obviously increased in three experiment groups and high-dose of HGAG can increase the number of red blood cells and platelets markedly. EachHGAG dose group also had significant effect on raising the number of bone marrowcells and the spleen index, lifting the G0/G1phase cell cycle arrest and thenaccelerating the proliferation of bone marrow hemopoertie cells.MTT assay was used to evaluate the effect of HGAG on BMNC proliferationby in vitro. By comparing the effects of HGAG on BMNC proliferation under thecondition of serum-free and10%serum, it was found that10%serum conditions canbetter reflect the ability of HGAG in promoting the proliferation of BMNC. Then theeffects of different molecular weighs HGAG on BMNC proliferation were observedunder the condition of10%serum. The results indicated the activities of HGAG onstimulating the proliferation would increase with the decrease of the molecular weightof HGAG, but required a higher concentration.To investigate the effect of HGAG on the proliferation ofgranulocyte-macrophage colony-forming unit (CFU-GM), erythrocytecolony-forming unit (CFU-E, BFU-E) and megakaryocyte colony-forming unit(CFU-Meg) with the method of hematopoietic progenitor cells culture technology inanemia mice in vitro. The results showed that the medium-and high-dose group canincrease the number of CFU-E, BFU-E and CFU-Meg markedly. No significant effecton the proliferation of CFU-GM was observed. WBC was derived from thedifferentiation of granulocyte progenitor cells, combined with the increase the numberof WBC by HGAG administered in vivo, it was assumed that HGAG can promote theproliferation of CFU-GM indirectly by promoting secretion of hematopoietic growthfactors in vivo. |
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