Correlation Between The Different Phenotype Of HRCT And Histone Deacetylase Activity In Patients With Chronic Obstructive Pulmonary Disease

Objective To explore the relationship among the peripheral blood mononuclear cellshistone (histone deacetylase, HDAC), the activity of systemic inflammatory reactionmedium interleukin-8(interleukin-8, IL-8) and the level and lung function of patientswith the different high resolution CT phenotype.Method Select62patients with acute exacerbations of COPD(AECOPD) and20healthy smoking people. All patients completed the COPD self assessment test (COPDassessment test, CAT) questionnaire, pulmonary function test, and high resolution CT(High Resolution CT, HRCT). According to the extent of emphysema and bronchialwall thickening in HRCT imaging, all patients of AECOPD were divided into threephenotypic. Type A: without emphysema or the existence of mild emphysema,irrespective of whether complicated with bronchial wall thickening; type E: thepresence of emphysema, not associated with bronchial wall thickening; type M: bothemphysema and bronchial wall thickening, and compare three kinds of phenotype andthe clinical data and lung function index. Separate peripheral blood mononuclear cellsand serum of all subjects, extraction of mononuclear cell nuclear protein. ELISA wasused to detect the activity of peripheral blood mononuclear HDAC and the level ofserum IL-8. Then compare the HDAC activity and IL-8levels between patients withthree group.Results (1) All patients according to the imaging manifestations of HRCT aredivided into3types, type A18cases, type E21cases, type M in23cases, nodifference between the groups of patients with age, gender, smoking index;(2) Patients with type A BMI index was significantly higher than that of E type and Mtype (P <0.05), CAT score was significantly lower than that of E type and M type (P<0.05);(3) Patients with type A FEV1/FVC value and FEV1, the percentage ofpredicted value, was higher than that of E type and M type (P <0.05); patients withtype A RV/TLC was significantly lower than that of E type and M type (P <0.05),patients with type E FEV1percentage higher than that of M genotype (P <0.05);(4)The COPD activity in patients with acute exacerbation of HDAC was significantlylower than that in the healthy control group (P <0.05), while the IL-8level wassignificantly higher than that of the control group (P <0.05); Patients of type A theHDAC activity was significantly higher than that of E type and M type(P <0.05),while IL-8was significantly lower than that of E type and M type;(5) Theactivity of HDAC is negatively correlated with IL-8and score of emphysema (r=-0.73，P＜0.05；r=-0.44，P<0.05), emphysema score was positively correlated withIL-8(r=0.77，P＜0.05), but no correlation between HDAC activity and bronchial wallthickening(P＞0.05).Conclusion The patients of sever emphysema are poor quality of life, lower bodymass index, ventilation function decreased significantly, systemic inflammation ismore severe, negative regulatory factors and histone deacetylase for systemicinflammation in patients withAECOPD.