Kidney Increasing Influence On The Film Side COH M Ouse Endometrial LIF And Expression Of Integrin β3

Objective: Experimental study， mice were observed controlled ovarianhyperstimulation keep increasing role in the film side window of implantation of eggsunder their mouse endometrium leukocyte inhibitory factor and integrin β3understandthe improvement of endometrial receptivity effects，and to explore the mechanism ofraising additional egg membrane parties in improving aspects of endometrialreceptivity．Methods: This issue will not conceive healthy female mice were randomlydivided into five groups：control group (saline group)，model group (GnRHa+HMG+HCG group)，positive control group (GnRHa+HMG+HCG+aspirin group)，lowdose group (GnRHa+HMG+HCG+dependent increase in egg membrane side lowdose group)，high-dose group (GnRHa+HMG+HCG+dependent increase in eggmembrane high dose group)．The third day began in the estrous cycle in mice，modelcontrol group，positive control group，low-dose group and high-dose groups werewithin the regular daily9：00intraperitoneal injection Barelin40μg/100g weight for9days．The first nine days while injecting HMG40u/100g weight，48h after injectionHCG100u/100g weight (control group while giving an equal volume of salineintraperitoneally) to construct a mouse model of controlled ovarianhyperstimulation．Build models while the positive control group, low-dose group，high dose group were given aspirin, keep the egg membrane increases low dosage ofliquid (equivalent to10times the adult dose)，raising the egg membrane increasedhigh dose of liquid (equivalent to20times the adult dose) fed with blank controlgroup and model control group were given a volume of0.9%sodium chlorideinjection，etc．intragastric gavage for11days. The mice were observed after injectionHCG48h vaginal smears，a large number of keratinocytes appear illustrate successfulmodel．In the injection HCG48h (window of implantation in mice)，the mice in eachgroup were killed，the uterine tissue in the middle，the middle of the uterine tissue samples were placed in sterile vials stored with4%paraformaldehyde fixedspecimens． The test subjects using immunohistochemical assay of leukemiainhibitory factor (leukemia inhibitory factor，LIF) and integrin β3．Results:1. In the expression of LIF：Compared with the control group，model controlgroup LIF protein expression was significantly lower than the control group，thedifference was statistically significant (P<0.05)：compared with the model group，positive control group low-dose group, the expression of LIF protein in the high-dosegroup were significantly higher than the model control group，the difference wasstatistically significant (P<0.05)：compared with the positive control group，low-dosegroup and high dose group of mice with LIF protein was significantly higher than thepositive control group，the difference was statistically significant (P<0.05)：low-dosegroup compared with the high-dose group， the difference was not statisticallysignificant (P=0.065>0.05)．2. In the expression of integrin β3：Compared with the control group，theexpression of integrin β3endometrial model control group was significantly lowerthan the control group，the difference was statistically significant (P<0.05)：with themodel group，the expression of positive control group，low-dose group，high dosegroup was significantly higher than integrin β3model control group，the differencewas statistically significant (P<0.05)：compared with the positive control group，low-dose and high-dose groups mice expressing endometrial integrin β3protein weresignificantly higher than the positive control group，the difference was statisticallysignificant (P<0.05)； low-dose group compared with the high-dose group， thedifference was not statistically significant (P=0.076>0.05)．Conclusion:Keep eggs increase membrane party can improve the COH mice planting windowperiod endometrial LIF and integrin beta3protein expression level, can improve theendometrial receptive of COH mice, its effect is better than that of aspirin.