The Status Of TAMs And The Expression Of TNF-α And EGFR In NSCLC

Objective:Lung cancer is one of the most deadly malignant tumor in China[1],whichis the leading cause of death from cancer in men. According to statistics,currently the NSCLC（Non-small-cell carcinoma,NSCLC）became the top ofthe malignant tumor,which including the morbidity and mortality. Nowadays,there are many pathogenesis of lung cancer, such as tumor gene mutation, therelease of inflammatory mediators and lymph node metastasis, etc. With thedevelopment of molecular biology, currently the relationship between theinflammatory mediators and tumor factors has become the research hotspot.The inflammatory mediators which associates with tumor mainly includestumor associated macrophages(TAMs), lipopolysaccharide (LPS), tumornecrosis factor-α(TNF-α),and interleukin family, etc. TAMs is one of themacrophages,when the peripheral blood mononuclear cell infiltrates into thesolid tumor tissues then it has been evolved. It can release a variety ofinflammatory factors, such as interleukin-10(IL-10), transforming growthfactor-β(TGF-β) and TNF-α,etc. TNF-α is produced by the active T cell incellular immunity and tumor immunity. TNF-α plays adouble-edged sword intumor. a high concentration TNF-α with interferon can strengthen the effect ofmacrophage activation to kill the tumor cells,and the low concentration ofTNF-α can induce the tumor growth and invasion.In recent years, theemerging of tumor factors include epidermal growth factor (EGFR), vascularendothelial growth factor (VEGF),micro RNA (miRNA) and B-celllymphoma-2(BCL-2),etc.EGFR is ubiquitously expressed in skin cells andstromal cells, and it is high expression in many malignant tumors.It ismediated signals including proliferation, migration, cell differentiation and the stability of internal environment, etc. EGFR is closely related to cellregeneration and plays important roles in the initiation and progression ofmalignancies. It is not difficult to draw that,the factor plays an important rolein the occurrence and development of tumor. Studying has suggested aroundthat, about three-quarters of patients who suffered with lung cancer.The EGFRhas a high expression in their tissues.At present,the method which in advancedNSCLC patients by (EGFR-Tyrosine Kinase Inhibitors,EGFR-TKI) combinedchemotherapy has been widely used.According to reports, about23%ofadvanced NSCLC patients who relapses quickly by interrupting EGFR-TKIdrugs[2]. Experiments have confirmed that inflammatory cells are activated,can release a lot of reactive oxygen species, leading to cancer[3].Byestablishing mice model, such as the colon cancer microenvironment of EGFin mice have a certain aggressive and initiative, in addition to the immunefunction of EGF, cancer cells by macrophages regulating invasive decline[4].Macrophages, TNF-α and EGFR are widely reasched in many tumors but thecombination testing of them in domestic at present has few studys. Theobjective of this experiment is to study the relationship between inflammationand cancer, and discuss the biological behaviour between them. Therefore,thegenesis of the tumor may result from interaction of inflammation andtumor.To provide the basis for inflammation medium which as the core ofcancer research.Methods:50case of patients with NSCLC which from paraffin embed constitutionwere used as experimental group.To choose the tissues which above the tumor3～5cm as control group.Using the immunohistochemical SABC method todetect The status of TAMs, and the expression of TNF-α and EGFR inNSCLC and normal lung tissue. With SPSS17.0to analyse the data, χ2test andSpearman rank correlation test were used as correlation analysis.Results:1.The results of TAMs in this exprement:Results The positive expressionrate of TAMs in NSCLC tissues and tumor-adjacent tissues was 56.0%(28/50) and28.2%(11/39).There was obvious differencebetween two groups (P<0.05). The expression was not associated withhistological type, differentiation degree and lymph node metastasis.2.The results of TNF-α in this exprement:The positive expression rate ofTNF-α in NSCLC was60.0%(30/50)�'�33.3%(13/39)). There wasobvious difference between two groups (P<0.05). The expression was notassociated with histological type, differentiation degree and lymph nodemetastasis.3.The results of EGFR in this exprement:The results of EGFR in thisexprement:Results the positive expression rate of EGFR in NSCLCtissues and tumor-adjacent tissues was70.0%(35/50) and33.3%(13/39).There was obvious difference between two groups(P<0.05). Results the positive expression rate of EGFR in poorlydifferentiated group and differentiated group was41.2%(14/34)、87.5%(14/16).There was obvious difference between two groups(P<0.05). Results the positive expression rate of EGFR in the lymph nodemetastasis and non-lymph node metastasis was74.1%(20/27)、43.5%(10/23).There was obvious difference between two groups(P<0.05). The expression was not associated with histological type.4.In NSCLC tissues,the expression level of TNF-α was positivelycorrelated with EGFR（r=0.356,P=0.011）.Conclusions:Using the SABC immunohistochemical method to test the status ofTAMs,the positive expression of TNF-α and EGFR in NSCLC andtumor-adjacent tissues, the results show points as follows.1. The TAMs has high expression in NSCLC tissues and the expression wasnot associated with histological type, differentiation degree and lymphnode metastasis.2. The TNF-α has high expression in NSCLC,it plays a key role in theoccerrance and development of NSCLC.3. EGFR has high expression in high malignant degree and lymph node metastasis.It shows that the prognosis poorer in the patients who has highexpression.4. In NSCLC tissues, the expression level of TNF-α was positivelycorrelated with EGFR may be a phenomenon.