The Study On The Resistance Of Helicobactance Pylori108Beads In Chengde Area

Objective:Helicobacter pylori (helicobacter pylori, H. pylori) is a microaerobicgramnegative bacilli. The world average of about50percent of people in-fected in the country and even reached60%. Numerous studies provedwith chronic gastritis, peptic ulcer, gastric mucosa associated lymphoidtissue (MALT) are closely related, In1994World Cancer Research instit-utions listed it as Class I carcinogen.H. pylori is very stubborn, once infe-cted, such as non-adult lifelong involvement will take regular treatment,that healing rate close to zero.Since H. pylori was found30years after adult infection has been con-firmed by regular treatment can completely eradicate the infection. This issomething worthy of people around the world rejoiced. However, withseveral large-scale eradication of promotion and study treatment showedthat the current preferred solution as the standard first-line triple therapy-PPI+two antibiotics to eradicate lower or much lower than the rate of80%, while H. pylori infection as a commuicable disease treatment ofchoice for efficiency should be higher than90%, otherwise such treatme-nt can not be used as the preferred solution. H. pylori eradication rate ofdecline was mainly due to increased rate of bacterial antibiotic resistance,due to the different areas of economy, culture, different healthcare levelslead to antibiotic resistance in various regions is also different. At present,for how to deal with the growing problem of antibiotic resistance has thefollowing two programs, one for the strict implementation of H. pylorieradication therapy and improve the range of sensitivity test, select thesenstivity to drugs based on susceptibility test results. The second is thediscovery of new therapeutic drugs that can be used to eradicate. By stud- ying Chengde Helicobacter pylori susceptibility results, draw relevant an-tibiotic resistance, and give some guidance in clinical medicine.Method:Collected during October2013to March2014due to the improvem-ement in gastrointestinal symptoms Chengde Central Hospital endoscopyroom endoscopy diagnosed as chronic gastritis with or without erosions,peptic ulcer, gastric cancer250cases of rapid urease-positive the patientsin this study (two weeks before the test is not taken antibiotics, bismuth,acid-suppressing drugs, etc.). Consent by patients taking gastric tissue gr-inder specimens were processed in the endoscopy seeded in H. pylori iso-lated and cultured on a special medium, cultured for3days at a constanttmperature micro-aerobic environment. By colony morphology, Gram sta-ining, oxidase test, catalase test, obtained after urease test identified108Parl H. pylori clinical isolates. Application Kirby-Bauer disk diffusion m-ethod were susceptibility to amoxicillin/clavulanate potassium, amoxici-llin, metronidazole, furazolidone, clarithromycin, levofloxacin, tetracycli-ne, minocycline, ampicillin antibiotic-line drug susceptibility9trials. Fin-ally,108Pearl H. pylori clinical isolates of nine kinds of different antibio-tic sensitivity of the analysis, suggesting that H. pylori Chengde higher s-ensitivity to antibiotics which can recommend clinically relevant antibiot-ic eradication treatment options to improve the initial treatment eradicate-on rates.Result:1. The250cases of mucosa specimens obtained experimentally bycultured108clinical isolates of H. pylori, culture-positive rate was43.2%.2. Culture-positive rate was40.0%male, female culture-positive ratewas48.0%.3. In108Hp culture positive specimens, the clarithromycin resistan-ctance rate of9.3%(10/108), amoxicillin, amoxicillin/clavulanate potas-sium resistance rate of4.6%(5/108) metronidazole resistance was76.9% (83/108), resistance rates furazolidone18.5%(20/108), levofloxacin resi-stance rate of9.2%(7/76), tetracycline resistance8.3%(9/108), ampicilli-n resistance was27.8%(40/108), minocycline resistance rate of12.0%(13/108).4. Chengde H.pylori existence of multiple drug resistance to metron-idazole and clarithromycin-resistant strains of five were for4.6%. Clarit-hromycin and amoxicillin while resistant strains of5,4.6%. Levofloxacinand clarithromycin-resistant strains of five simultaneously, was4.6%. Bo-th drugs were found simultaneously resistant beads15, was13.9%. Not f-ound on three or more antibiotic-resistant strains.Conclusion:1. Chengde H.pylori to amoxicillin, amoxicillin/clavulanate potass-ium, clarithromycin higher sensitivity may continue as the preferred med-ication.2. Chengde H.pylori tetracycline, doxycycline if rice, furazolidone s-ensitivity is higher, taking into account the absorption rate, side effects, isnot suitable as the preferred medication.3. Chengde metronidazole resistance was76.9%, still higher, and nocorrelation with the type of disease.4. Chengde area ofculturpositive rate of H.pylori no significant corr-elation with gender.5. Chengde by rapid urease test positive for H.pylori infection intissue culture positive rate was43.2%, with an average of30-50%of cult-urepositive rate was no significant difference.6.H.pylori Chengde area of the existence of multiple drug resistance,proper selection of suitable drugs can improve eradication rates.