An Application Of Embryonic Skin Cells To Repair Diabetic Skin Wound: A Wound Reparation Trail

Background: Cell therapy has shown its power to promote diabetic chronic wound healing.However, problems of scar formation and loss of appendages have not yet been solved. Ourstudy aims to explore the potential of using embryonic skin cells (ESkC) to repair diabeticwounds.Methods: Circular wound was created on the back of the diabetic mice, and ESkCs stainedwith CM-DIL were transplanted into the wound. Wound area was recorded at the day4,7,1114after transplantation. The tissue samples were obtained at week1,2and3, and tissue sectionswere stained by TGF-β1, TGF-β3, VEGF and CD31. The new skin formed on the wound of thediabetic mice with ESkC treatment at week1but not on the wounds of the non-treatment group.Results: The histological scores of diabetic group with ESkC treatment were significantlybetter than the non-treatment group (p<0.05). The fluorescence examination of CM-DIL andCD31staining indicated that the ESkCs participated in the tissue regeneration, hair folliclesformation and angiogenesis. The expression of TGF-β1and VEGF in ESkC treated groups wasnoticeable in week1, but disappeared in week2. TGF-β3was not expressed at week1, butexpressed markedly around hair follicles in week2in ESkC treated groups.Conclusion: Our study demonstrated that ESkCs are capable of developing new skin withappendage restoration to repair the diabetic wounds.