| Objective:To investigate the effects of fructose-1,6-diphosphate preconditioning on acute lung injury induced by liver ischemia-reperfusion in rats.Methods:Twenty-four healthy male SD rats were randomly divided into3groups and each group had6rats. In Control group(S group), only abdominal surgery was operated after blood vessels were dissociated. Cold ischemia-reperfusion was performed in Model control group (M group) without any treatment. In the fructose-1,6-diphosphate group (FP group), the model was the same as M group, while FDP250mg/kg was injected in the tail vein15min before cutting the skin. Twenty hours after reperfusion, bronchoalveolar lavage fluids were collected to detect the level of TNF-a、IL-6、IL-10、NO. Meanwile, lungs were removed to detect the level of MDA and SOD in tissue homogenate. The pathological changes of lung were evaluated by light microscopy. Real-time PCR was used to detect the level of iNOS mRNA.Results:Compared with S group, the level of MDA, TNF-a、IL-6and NO rised statistically in M group and F group (P<0.05), the level of SOD and IL-10decreased statistically in M group and F group. Compared with M group, the level of MDA, TNF-a、IL-6and NO rised statistically in F group (P<0.05), the level of SOD and IL-10decreased statistically in F group (P<0.05). There were no obvious morphologic changes in S group. Light microscope showed that plenty of inflammatory cells infiltration, the obvious exudate in narrow alveolar space, the significant bleeding and hyperemia in pulmonary intersitital tissue, the thickening of alveolar interval, while it was more slight in F group. The results of real-time PCR showed that the expression of iNOS mRNA in S group were significantly lower than M group and F group (P<0.05), while compared with the control group, the expression of iNOS mRNA in F group were lower than M group (P<0.05).Conclusion:Fructose-1,6-diphosphate preconditioning can obviously attenuate acute lung injury induced by liver cold ischemia-reperfusion in rats. |
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