STAT3, TGF-β1on The Regulation Of Pathogenesis In SLE And Clinical Relevance Of Patients With SLE

Objective:SLE (systemic lupus erythematosus, SLE) is characterized by producing a variety of autoantibodies, activation of complement system and deposition of immune complex, and then leads to the damage of tissue and organ, morever, whose etiology and pathogenesis is not entirely clear. Recent research suggests that immune dysregulation plays a dominant role in the pathogenesis of SLE, which throughout the course of the disease, including abnormal regulation of immune cells. However, the roles of immune cells are achieved by intracellular signal transduction, What’s more, STAT3and TGF-β1play an important role in the pathogenesis of SLE. To study STAT3, TGF-β1regulation of SLE and clinical relevance of patients with SLE,our discussion as follows:1. the expression difference of STAT3and TGF-β1in health adult and SLE patients;2. the expression difference of STAT3and TGF-β1between male and female in SLE patients.3the expression difference of STAT3and TGF-β1between active group and inactive group, inactive group and normal control group in SLE patients;4. the correlation between the expression of STAT3and TGF-β1mRNA and clinical manifestation;5.the correlation between the expression of STAT3and TGF-β1mRNA and autobodies;6. the correlation between the expression of STAT3and TGF-β1mRNA and SLEDAI. Which provide a theoretical basis for clarifying pathogenesis, monitoring of disease activity and drug exploitation of SLE. Method:RT-PCR method was used to analysis the expression quantity of STAT3, TGF-β1mRNA in SLE patients (40cases) and health adults (40cases), The PCR products were electrophoretic by2.0%agarose gel, and then confirmed by gel imager camera.According to the diagnostic criteria, statistical software was used to analyze the expression of STAT3, TGF-β1and their correlation with clinical characteristics.Result:1. The expression level of STAT3and TGF-β1mRNA in peripheral blood of SLE patients were fond to have significantly higher than normal controls (P<0.01).2. The mRNA expression level of STAT3and TGF-β1in peripheral blood of SLE patients were not found to be different between male and female SLE patients (P>0.05).3. The mRNA expression level of STAT3and TGF-β1in peripheral blood in active group is obviously higher than inactive group (P<0.05), the levels of STAT3and TGF-β1mRNA in inactive SLE group were significantly higher than the normal control group (P<0.05).4. The levels of STAT3mRNA in SLE patients with fever group were significantly higher than the SLE patients without fever group (P<0.05).5. The expression level of STAT3and TGF-β1mRNA show a positive correlation with SLEDAI (P<0.05); there’s no correlation between the mRNA expression level of STAT3and TGF-β1(P>0.05).6. There’s no correlation between the expression level of STAT3and TGF-β1mRNA and autoantibodies(P>0.05).Conclusion:1. The expression level of STAT3and TGF-β1mRNA in peripheral blood of SLE patients is elevated than normal control which suggested that STAT3and TGF-β1may play a role in the pathology of SLE.2. The expression level of STAT3, TGF-β1mRNA in peripheral blood of SLE patients in active group were obviously higher than inactive group and showed a positive correlation with SLEDAI,which is to say STAT3, TGF-β1may be related with the activity of SLE and can be considered as SLE activity index.3. The expression level of STAT3mRNA in peripheral blood of SLE patients had a correlation with fever which suggested that STAT3may be involved in the process of fever.4. There’s no correlation between the expression level of STAT3and TGF-β1mRNA and which suggested that STAT3and TGF-β1maybe through different mechanism result in SLE.