| ObjectiveThrough this study of atherosclerosis in a mouse model for drug intervention to investigate the effects of atherosclerotic vulnerable plaque, as well as the mechanism of plaque angiogenesis by the Wenyanghuoxue detoxification prescription, and through clinical observation study to explore the improvement of TCM symptoms in patients with acute myocardial infarction by Wenyanghuoxue detoxification prescription, thus providing evidence-based medicine for the prevention and treatment of coronary heart disease by the Chinese Medicine.Methods1008-week-old male ApoE-/-mice were given a high-fat diet for13weeks to make the model, and were randomly divided into model group, simvastatin group, low-dose group of Chinese medicine, middle-dose group of Chinese medicine and high-dose-group of Chinese medicine after the model is success-ful, and were respectively given distilled water, simvastatin and Wenyanghuoxue detoxifying decoction with small, medium and large doses of treatment13weeks, and to observe the pathology of lord artery vulnerable plaque in each group by HE staining, Movat special staining and immunohistochemical method, and finally calculate the cellular components of arterial plaque and neovascul-arization in each group, and finally through statistical analysis to compare the effects of each group after administration.Clinical Study:30cases that meet the criteria of acute myocardial infarction patients were randomly divided into Wenyanghuoxue detoxifying herbs group (Chinese medicine combine with conventional western medicine) and comparison group (simple conventional western medicine),15cases in each group. Observed the TCM symptom score improvement in all groups of patients before and after treatment, and finally through statistical analysis to compare the therapeutic effects of traditional Chinese medicine and western medicine.Results1. After treatment in each group, compared with the model group, the plaque vulnerability index of the low dose group of Chinese medicine did not change significantly (P>0.05), while the simvastatin group, middle dose group and high dose group of Chinese medicine with a significant difference(P<0.05); Compared with simvastatin group, the middle dose group and high dose group of Chinese medicine to reduce plaque vulnerability index did not significant difference (P>0.05), descripting that the efficacy of middle dose and high dose of Chinese medicine with simvastatin in reducing plaque vulnerabili-ty indices considerably. The comparison between the groups of Chinese medicine, the middle dose and high dose group is more obvious than the low dose group in reducing plaque vulnerability index, the difference was statistically significant (P<0.05), while there is not different between the middle dose and high dose group in reducing plaque vulnerability index (P>0.05)。2. After treatment in each group, compared with the model group, this is not significant difference (P>0.05) in the low dose group of Chinese medicine to reduce the density of the plaque neovascularization, whereas the simvastatin group, middle dose group and high dose of Chinese medicine has significant difference (P<0.05); compared with simvastatin group, the middle dose group and high dose group of Chinese medicine has not significant difference (P>0.05) to reduce plaque neovascularization density, indicating that the the efficacy of middle dose and high dose of Chinese medicine with simvastatin in reducing plaque neovascularization density considerably. The comparison between the groups of Chinese medicine, the middle dose and high dose group is more obvious than the low dose group in reducing neovascular density, the difference was statistically significant(P<0.05), in which the high dose group was significantly effect of the middle dose group, the difference has statistical significance (P<0.05).3. In terms of clinical observation, the symptom scores of Wenyanghuoxue detoxifying herbs group and the comparison group were significantly lower before and after treatment, the difference was statistically significant (P <0.05); compare the comparison group, the total efficacy of TCM symptoms of Wenyanghuoxue detoxifying herbs grou was significantly up, the difference was statistically significant(P<0.05).ConclusionCertain concentration Wenyanghuoxue detoxification prescription like as simvastatin, are able to reduce the vulnerability index of mouse model of atherosclerosis and neovascularization density, and have a role in maintaining the stability of vulnerable plaque, the theoretical mechanisms may inhibit plaque angiogenesis, this need more in-depth study to confirm. Meanwhile, Wenyanghuoxue detoxification prescription combined conventional medicine treatment of acute myocardial infarction, better than medicine with western medicine in terms of symptom efficacy. |
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