| In this paper, the Mongolian medicine MenggenWusu-18was selected as research object. The Mongolian medicine were ground to fine powder, and separated and extracted three times with distilled water and simulated gastric juice. Then, the samples of original medicine, soluble and suspended state were digested using Microwave system.Then the contents of Cu, Fe, Mg, Zn, Mn, Cd, Sr, Cr, Al, As, Ni, Co, Ag, Pb and other trace elements were determined respectively by ICP-AES and the content of Hg by Double light digital mercury analyzer. The results showed that the contents of Ca, Fe, Mg, et al, which are beneficial to human, are relatively high, between1146.64～24334.55μg/g; the contents of Cr, As, Pb, et al., which are harmful to human, are relatively low, between4.39～9.51μg/g. But the content of Hg in MenggenWusu-18was up to about6143.66μg/g, which has exceeded the national standard. The situation is closely related to the fact that Hg is added in Menggen Wusu-18during processing to attain the objective.The soluble state of MenggenWusu-18was separated by different resins for morphological analysis. The methods of elemental analysis were the same as above. Through the calculation of elemental species distribution,the contents of Ca, Fe, Mg, Zn, Mn, et al., were higher in the free state than in the non-free state, higher in the inorganic state than in the organic state and higher in the steady state than in the non-steady state. The content of Hg was higher in the free state than in the non-free state, then the free state and the non-free state in simulated gastric juice existed with a ratio of4:6; The content of Hg was higher in the inorganic state than in the organic state; The content of Hg in the two kinds of extracts were higher in the steady state than in the non-steady state. In short, the content of Hg in the soluble state were very low and between9.5623×10-6～2.1552×10-4μg/g. The toxic effect of Hg in Mongolian medicine was researched by integrating chemical experiment with animal experiment. MenggenWusu-18with the normal dose and50times higher than the normal dose concentration were fed to two groups of mice, and a black group with no MenggenWusu-18in the meantime. By comparing their situation, anatomizing, collecting blood sample, taking out livers and collecting feces, the content of Hg determined after freeze-dried and digestion, and the pharmacokinetic parameters was calculated.The results showed that:①The content of Hg mice with normal dose in the blood was no different from the blank group between0.1034～0.6460μg/g. The Hg content of mice with high dose in the blood was relative higher than that with normal dose between0.1938～0.9173μg/g.②The content of Hg mice with normal dose in the liver was also no different from the blank group between0.2390～0.6179μg/g. The Hg content of mice with high dose in the liver was relative higher than that with normal dose between0.3740～2.2739μg/g.The content of Hg increased obviously over time in4h,12h,24h,48h, which reached2.2739μg/g in48h.③The content of Hg in the feces was higher. The Hg contents of mice with normal dose in feces were between0.7658～1.8109μg/g. The Hg contents of mice with high dose in feces were between8.7265～100.7800μg/g. The content of Hg in the feces increased over time.In short, the content of Hg in MenggenWusu-18had exceeded the standard. But it was initially confirmed by animal experiments that Hg had no significant effect on the health of the mice under normal dose. Hg was excreted without causing harm to the body; in the high-dose group, the majority of Hg was still excreted from the body, Part of Hg would accumulated in the blood and liver for period, however, most of the elemental Hg would be excreted over time, so it would not cause harm to the body. The results just was preliminary and need to be further studied. |
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