Study On The Protective Effect Of KFL On Radiation-induced Genetic Damage

In recent years,with the intense situation of the worldwide nuclear security and the increasingly widespread application of nuclear energy and radiation technology in the field of energy industrial and agricultural production, health care, scientific research and national defense, radioprotective agents are of significant importance in medical, industrial, environmental, military and space applications. More and more countries have increased value of the prevention and treatment of radiation damage. As an effective method to treat radiation injury, radioprotectants once again cause for concern.2,2-dimethylthiazolidine named as KFC is a new class of drugs that our institution developed with independent intellectual property rights. KFC is a white needle or rod crystal, with odor smell, being soluble in water, ethanol, methanol and insoluble in ether solvents such as tiny, with its melting point170-173℃. The Institute has established a quality control method for KFC and demonstrated that KFC has a significant anti-radiation protective effect through vivo experiments. Moreover, it can be able to selectively protect normal tissue. To further study the adverse effect of KFL on radiation-induced genetic damage, Chromosome aberration analysis, micro nucleus test and single cell gel electrophoresis were used to observe the protective effects of KFL on the genetic damage of human peripheral blood lymphocytes induced by radiation.To investigate the protective effect of KFL on radiation-induced genetic damage, the radiation-sensitive human peripheral blood lymphocytes was selected as the subject. Firstly, in order to determine the nontoxic dosage range of KFL on human peripheral blood lymphocytes, MTT assay was used to messure the effect of KFL on Human monocytes THP-1, and thus to select an appropriate dosage of administration for the follow-up test. Our study select the chromosomal aberration rates, the micronuclear cell rates, and the micronuclear rates, the tailleghth, the tailmoment, the olivetailmoment and the tail DNA%of comet cells as analysis of indicators to evaluate the protective effect of KFL on radiation-induced genetic damage.The test results show that compared with the control group, the chromosomal aberration rates, the micronuclear cell rates and the injury degree of DNA in the four KFL protective groups were all significantly decreased. That indicated a significant investigate the protective effect of KFL on radiation-induced genetic damage, which may be related to its role of antioxidant, scavenging free radicals. This investigation have provided an experimental basis for further study of the radioprotective mechanism of KFL, and also for its clinical applications.