Effects Of PAHs On No Occupation Exposure In Peripheral Blood AhR And TH1/TH2Type Cytokine

ObjectiveTo study the polycyclic romatic hydrocarbons (PAHs) exposure levels and influencing factors of non-occupation crowd in Tianjin by epidemiology investigation and biological surveillance. To study the expression of PAHs’metabolites-1-hydroxypyrene (1-OHPyr) in the aryl hydrocarbon receptor (AhR) in peripheral blood and TH1/TH2type cytokines. Then to discuss the mechanism of immune infury of PAHs’low-dose and long-term exposure.MethodsThis study collected67healthy volunteers from a physical examination center in Tianjin in May2012as a research object, the volunteers all lived in Tianjin for more than5years, collected some basic informations such as life style and so on from the research object by the means of conducting a questionnaire after informed consent. Alkaline hydrolysis-HPLC method and enzyme-linked immunosorbent assay were used as experiment measures to determine the concentration of1-Hydroxypyrene in human urine and the express level of TH1/TH2type cytokines. SPSS17.0were used as the statistical software to analyse the correlation relationship between the concentration of1-OHPyr in human urine and the expression of TH1/TH2type cytokines. Combined with the epidemiological data, we used independent samples t test, analysis of variance in accordance with normal distribution, otherwise, we used nonparametric test and logistic regression analysis to analysis.ResultsIn this study,1-OHPyr in human urine in Tianjin non-occupation crowd was ranged from0.21to6.70μmol/mol Cr, the median concentration was1.13μmol/mol Cr. The1-OHPyr content in female (1.22μmol/mol Cr) was higher than male (0.80μmol/mol Cr), but the difference had no statistically significant (P＝0.07). The urine1-OHPyr in non alcoholic was higher than alcoholic (P=0.03). Smoking, passive smoking, eating fried foods, preserved foods and urinary1-OHPyr concentration levels did not have a statistically significant difference (P>0.05). By the analysis of multiple affecting factors of urinary1-OHPyr content, we realized that the use of mobile phones and microwave oven were risk factors to the urinary1-OHPyr content, namely, the using frequency was higher, the urinary1-OHPyr content was higher; at the same time, there were interaction on the urinary1-OHPyr content in using mobile phones and microwave ovens (P<0.05), suggesting that the electromagnetic radiation might have effects on PAHs exposure.The peripheral IL-2median concentration was13.71pg/ml, the peripheral IL-4median concentration was5.20pg/ml, the peripheral IL-10median concentration was180.76pg/ml, the peripheral IFN-y median concentration was176.23pg/ml, the peripheral AhR median concentration was0.13ng/ml. We used Kruskal-Wallish test to analyze the data and found that the concentration of IL-2, IL-4, IL-10, IFN-y and AhR showed no statistical significance at all (P>0.05). This investigation showed that when the urinary1-OHPyr was ranged from0.21to1.92μmol/mol Cr, IL-2and IL-4decreased gradually with the increase of1-OHPyr content, but there was no statistical significance (P>0.05). The content of IL-10in the1-OHPyr content in0.65～6.70μmol/mol Cr ranged with the increase of1-OHPyr content gradually increased, there was no statistical significance (P>0.05), The IFN-γ decreased in the1-OHPyr contents in0.65～6.70μmol/mol Cr ranged with the increase of1-OHPyr content, there was no statistical significance (P>0.05), but there were significant changes in IL-2concentration in the1-OHPyr content in0.65～1.13μmol/mol Cr group and the1-OHPyr content in1.14～1.92μmol/mol Cr group (P=0.08), we can increase the content of the sample to make a further analysis.The urinary1-OHPyr showed no correlation with the content of peripheral blood AhR (P>0.05). With the increased levels of PAHs exposure, changes of peripheral blood AhR concentration was not found to have statistically significant (P>0.05).With the gradual increase of urinary1-OHPyr concentration, TH1/TH2ratio change was not obvious, there was no significant difference (P=0.453). After we controlled the factors of sex, age, electromagnetic radiation and occupational chemical contact, the partial correlation analysis showed no statistical significance (r=-0.209,P>0.05).Conclusion The PAHs exposure levels were generally higher in Tianjin, there was interaction between the microwave oven and mobile phone changes of urine1-HOPyr in use, there was no effect between PAHs exposure levels and peripheral blood AhR content, but the exposure may have a effect on interleukin secretion. Urinary1-HOPyr is the most commonly used contact biological markers of occupational PAHs exposure, the detection method is simple, accurate and determinative, the marker will remain used in general population index in large-scale epidemiological investigation of biological monitoring. With the increased levels of PAHs exposure, the expression of TH1/TH2type cytokines ratio change was not obvious, this might be due to urinary1-OHPyr could not reflect the comprehensive PAHs exposure level, and the number of samples was not enough. To confirm PAHs exposure with TH1/TH2type cytokines, we can expand the content of the sample, use multiple PAHs exposure markers and biomarkers for joint detection to evaluate the PAHs exposure on body damage comprehensively and accurately, to analyze the influencing factors.