| Chinese tea culture has a long long history; the tea culture is highly diverse in China as a result of the rich biological, ethnic and cultural diversity. Except for Camellia sinensis, various plants are known to be used as drinking tea locally in different part of China, for example, Vine Tea (Ampelopsis grossedentata), Large-leaved bitter tea (Ilex latifolia, I.kudingcha), Small-leaved bitter tea (Ligustrum robustum), sweet tea (Rubus suavissimus, Lithocarpus polystachys) and Hawks tea (Litsea coreand), etc. Therefore, we define as "’non-Camellia Teas" those plants that (1) do not belong to Theaceae Camellia,(2) have been long and widely used to as drinking tea with a history of at least500years within an area spanning a minimum of a few counties and within an ethnic group, and (3) are being actively used nowadays and are readily available on the market. In this study, the antixodiant activities of thirty-three non-Camellia teas were evaluated by four different in vitro assays including1,1-Diphenyl-2-picryl-hydrazyl(DPPH) radical-scavenging activity, ferric-reducing antioxidant power (FRAP), ferrous ion-chelating (FIC) and (3-carotene-linoleate bleaching assay. In addition, the polyphenol contents of different teas were estimated by Folin-Ciocalteus assay. Vine tea showed a high antioxidant potential and was choosed for the further research.Herein, we summarized the modern research of vine tea. Vine tea is originated from Ampelopsis grossedentata (Vitaceae family) with Chinese name:"Teng" tea. It grows wildly in the provinces south of the Yangtze River such as Guangxi, Hunan, Hubei, and Guizhou and was extensive used locally with a very long history. It is brewed as tea for drinking mainly used for the treatment of icteric hepatitis, cold caused by wind-fire, sore throat, furuncle and carbuncle, alcohol intoxication and constipation, etc. and investigated the folk application, local production and trade situation of vine tea mainly in Hunan and Guizhou provinces.The chemical fingerprint of vine tea was established by UPLC-DAD. The antioxidant activity of each sample was evaluated by1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging activity. Then, the grey relational analysis for30batches of vine tea was performed based on the common peak area. The relationship between fingerprints of Ampelopsis grossedentata and the antioxidant activities were investigated. These results indicated that the antioxidant activity of the vine tea may be due to the synergistic effect of multicomponents, especially dihydromyricetin. At the same time, the contents of dihydromyricetin in40batches of vine tea were conducted by HPLC-DAD. The results indicated that the content of dihydromyricetin was different obviously in different areas without obvious regulation, the maximum was23.08%and the minimum was5.07%. This may be explained by interaction of environment, harvesting time, processing methods or several different mechanisms.Finally, the brewing conditions on the antioxidant activity of a cup of vine tea (Ampelopsis grossedentata) were optimized, different extraction parameters such as temperature, solid-liquid ratio; time and frequency, kind of water on the DPPH radical scavenging rate of vine tea were investigated by Box-Behnken design-response surface method. The results showed that the optimum brewing conditions for vine tea were as follows:temperature80℃, time5.8min and solid-liquid ratio1:56(g/mL), then the DPPH radical-scavenging activity of vine tea reached the best. The research provides theoretical guidance and reference for scientific drinking way of vine tea.In summary, this thesis mainly foucs on the investigation of the folk application, marketing and antioxidant activies of vine tea,to provide theoretical evidence for the further development of the medicinal value in vine tea, and also be useful for the rational utilization of this herbal medicine resource. |
PDF Full Text Request