| Objective:Chronic heart failure is a common clinical syndrome, which is a progressive, fatal disease of the heart muscle. Chronic heart failure (CHF) represents an inflammatory state of chronic stress, injury and ischemia for cardiac myocytes. Ventricular remodeling and myocardial fibrosis play an important role in the genesis and maintenance in CHF. Ventricular remodeling is pathophysiological basis of CHF. HSPs are called stress proteins because they are produced under stimulation such as heat, cold, ischemia, infection and so on,. Heat shock protein47(HSP47) is a highly conservative proteint, it recognized as the pro-collagen-specific chaperone protein and play an important role in the process of collagen deposition and fibrosis, The thesis is aimed at studying the expression of HSP47in atrial tissues with Real-time Fluorescence Quantitative PCR(FQ-PCR) and its relevance to P I CP, which is the indexs of myocardial fibrosis in blood-serum, and to explore the molecular mechanisms of structural ventricular remodeling.Methods:The right atrial tissues and blood-serum samples were taken from60patients with cardiac surgery.20patients were in NYHA Igroup,20patients were in NYHA Ⅱ group,20patients were in NYHAⅢ group. The mRNA amounts of HSP47were studied by Real-time Fluorescence Quantitative PCR method, and the concentraili-ons of carboxyterminal propeptied of type Ⅰ procollagen in serum were measured by enzyme-linked immunosorbent assay (ELISA).Results:(1).The mRNA level of HSP47significantly increased in both NYHA Ilgroup and NYHA Ⅲ group compared with NYHA Igroup (P<0.05, P<0.05). The mRNA level of HSP47significantly increased was also observed in the NYHA III group compared with NYHA Ⅱ group (P<0.05).(2) The data mRNA expression of HSP47and the content HSP47in blood-serum have a significantly positive correlation.(3). Compared with NYHA Ⅰ the concentration of serum P I CP and left ventricular diameter were significantly increased, left ventricular ejection fraction were significantly decrease in NYHA Ⅱgroup(P<0.05, P<0.05, P<0.05).The significantly increased was also observed in the NYHA Ⅲ group compared with NYHA II group in the concentration of serum P I CP and left ventricular diameter, left ventricular ejection fraction were significantly decrease in NYHA Ⅲ group (P<0.05, P<0.05, P<0.05).(4)There was a conspicuous and independent direct correlation between the expression of HSP47and the P I CP and left ventricular diameter (r=0.47,P<0.01; r=0.785.P<0.05)Conclusions:(1) There are myocardial fibrosis and incereased left ventricular diameter in patients with chronic heart failure. And there is a significantly positive correlation between the myocardial fibrosis and left ventricular diameter.The myocardial fibrosis plays an important role in ventricular remodeling.(2)The HSP47gene highly expresses in chronic heart failure and closely correlated with the degree of disease severity. It indicates that HSP47gene may be involved in the ventricular remodeling.(3)The expression of HSP47in atrial tissues are correlated between P I CP and left ventricular diameter, which demonstrated that the expression of HSP47may be one of the mechanisms of myocardial fibrosis and it is closely related with structural ventricular remodeling. |
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