The Investigation Of Preventive Mechanism Of Depfessive Position With Sertraline In Ischemic Stroke Rats

BackgroundPost-stroke depression (PSD) is a frequent complication of stroke, The cognitive impairment and the mood disorder are commom symptoms of PSD. Using antidepressants after stroke to prevent depression to improve the quality of life for patients, promote significant neurological rehabilitation. Tyrosine kinase receptor B (TrkB) is one of receptor of brain-derived neurotrophic factor (BDNF), cAMP response element binding protein (CREB) is regulator of Signal transduction pathway. Their changes are possible by the expression of BDNF and indirectly affecting the PSD changes occur.Object1To observate change the behavior of rat model of cerebral ischemia using sertraline, realizing the effect of sertraline for depression occurs of rat model of cerebral ischemia.2To determine expression of TrkB and CREB in PSD rats hippocampus, consulting the effect of TrkB and CREB on the prevention and treatment of PSD.Methods1The complete cerebral ischemic rat model is set up by occluding the bilateral carotid artery after improvement. On this basis, further isolated-living condition and chronic unpredictable mild stress (CUMS) are applied successively to set up the PSD rat model. The experimental rats were given sertraline by gavage to simulate patients with oral sertraline.2To measure the weight of rats and to observate the trend of weight change.To test the interesting and response capability of rats by sucrose consumption test. To test the ability to independently explore and the Spontaneous movement capability by open field test (OFT). To evaluate rats model’s depression by the resulting data.3To detect expression of TrkB and CREB protein in the hippocampus of PSD rats by heTusing immunohistochemistry technique. Expressions of TrkB and CREB mRNA in the hippocampus of PSD rats are detected by using reverse transcription-polymerase chain reaction (RT-PCR). consulting the effect of TrkB and CREB on the prevention and treatment of PSD.Results1Test of behavior①The PSD group and depression group showed significantly less weight compare with control group and sertraline group. On the7th,14th,21th after CUMS, the differences have statistical significance (P<0.01or P<0.05); The sertraline group compared with the normal control group, there is no statistical significance (P>0.05).②Compared with the normal control group and sertraline group, the PSD group and depression group showed significantly less sugar-water consumption On the7th,14th,21th after CUMS, the differences have statistical significance (P<0.01); The sertraline group compared with the normal control group, there is no statistical significance (P>0.05).③PSD group and depression group showed significantly less weight OFT data compare with control group and sertraline group. On the7th,14th,21th after CUMS, the differences have statistical significance (P<0.01or P<0.05);Compared with the normal control group, the sertraline group OFT data no statistical significance (P>0.05).2Immunohistochemistry④TrkB protein levels of the PSD group and depression group were significantly declined, the differences have statistical significance (P<0.01) compared with the control group, and the sertraline group were significantly increased (P<0.01) compared with the PSD group. ②CREB protein levels of the PSD group and depression group were significantly declined (P<0.01) compared with the control group, and the sertraline group were significantly increased (P<0.01) compared with the PSD group.3RT-PCR①TrkB mRNA levels of the PSD group and depression group were significantly declined compared with the control group, the differences have statistical significance (PPSD=0.010, PD=0.021); sertraline group were significantly increased compared with the PSD group, the differences have statistical significance (Pser=0.023).②CREB mRNA levels of the PSD group were significantly declined compared with the control group, the differences have statistical significance (PPSD=0.011); sertraline group were significantly increased compared with the PSD group, the differences have statistical significance (Pser=0.012).Conclusions①Prevention of stroke in rats using sertraline may reduce the incidence of PSD.②The expression of TrkB and CREB of sertraline group in hippocampal have increased, may involvement in the prevention and treatment of PSD.