The Research Of Three Bacteria Detection With The Clinical Characterization In Root Canal Treatment

Purpose: Detection of root canal treatment the patients root canalread white staphylococcus, streptococcus mutans, the archaea four bacteriadetection rate, and analyze the root canal treatment patients again read whitestaphylococcus, streptococcus mutans, the archaea three kinds of bacteria andthe relationship between the clinical symptomsMethods:Eextraction Enterococcus faecalis standard strains totalRNA genome, the total RNA genome DNA enzymes processing, determinationof total RNA genome concentration, through the retrovirus PCR will RNAretrovirus get cDNA, gradient degree evaluation Real-time PCR technologydetermination dung bowel coccus mRNA expression level of thevalue.Clinical selection of131patients with root canal root canal treatmentagain89with teeth (sichuan university huaxi hospital oral), through therecords of dental clinical related information such as signs, symptoms, tooriginal fillings, grubbing tube inserted into the sterile paper point60seconds,With H file scrape content in EP tube, extraction of genomic DNA, PCRreaction tests read white staphylococcus, streptococcus mutans, the archaea,four bacterial16s RNA detection of root canal treatment the patients rootcanal read white staphylococcus, streptococcus mutans, the archaea threebacteria detection rate, Analysis of root canal treatment patients again readwhite staphylococcus, streptococcus mutans, the archaea four types ofbacteria and the relationship between the clinical symptoms. The results forthe statistical analysis using SPSS13.0software to analysis of variance.Results: Detection of root canal treatment the patients rootcanal read white staphylococcus, streptococcus mutans, the archaea four bacteria detection rate is respectively:31.46%,29.21%,19.1%. Knockingpain in the clinical symptoms of streptococcus mutans in case group casedetection rate is higher than that in without knocking pain group (P <0.05).Show that detection of streptococcus mutans is closely related to theclinical symptoms of knocking pain, and periapical tissue damage werepositively correlated.Conclusion:1Root canal treatment the patients root canal can detect read whitestaphylococcus, streptococcus mutans, the archaea three kinds of bacteria.2Root canal treatment the patients root canals of streptococcus mutans is closelyrelated to the knock detection and clinical symptoms of pain, and periapical tissuedamage were positively correlated.