The Effectiveness Of Huoxue Huayu Prescription On Bleomycin Induced Pulmonary Fibrosis In Rats

Background and ObjectivesIdiopathic pulmonary fibrosis is an unexplained, chronic, irreversible lung disease withlittle treatment option. Its pathological features appear as usual interstitial pneumonia.The traditional Chinese Medicine Classifies IPF as ‘Feibi’ or ‘Feizhang’, which could begiven promoting blood circulation and removing blood stasis therapy, and achievessome effects. In this thesis we are going to confirm the therapeutic validity of HuoxueHuayu Prescription, evaluate whether conbined treatment of traditional Chinese herbsand chemical drugs can improve the effectiveness, then explore the possible mechanismof Huoxue Huayu Prescription on pulmonary fibrosis.Methods50rats are randomly divided into5groups:①normal control group (Group CTRL);②bleomycin model group (Group BLM);③traditional Chinese herbs group (Group CH);④pirfenidone group (Group PF);⑤combination of Chinese herbs and pirfenidonegroup (Group CP). Group BLM, Group CH, Group PF and Group CP are establishedbleomycin induced fibrosis models while Group CTRL uses saline as contrast. From theday after opration to day28, Group CH, Group PF and Group CP are intragastric ad-ministration with Huoxue Huayu Prescription, pirfenidone solution or both of them.Meanwhile, Group CTRL and Group BLM are given a gavage of saline. Then we gettheir coefficient of lungs and cut their pulmonaries into sections.After that use HE andMASSON coloration to assess the therapeutic effect. Western blot are applied to detectthe expression of ALK5, Smad2/3and EGFR in lungs. TBA kit is used to detect thecontent of MDA.ReslutsCoefficient of lung: Compared with Group CTRL, the coefficient in rest of groups rise invarying degrees(P＜0.05) while Group BLM, Group CH, Group PF and Group CP havelittle different from each other(P≥0.05). Ashcroft score on HE coloration: Compared with Group CTRL, the coefficient in rest ofgroups rise in varying degrees(P＜0.05). Compared with Group BLM, Group CH, GroupPF and Group CP decrease a certain level(P＜0.01), and Group CP has a further cut toGroup CH and Group PF(P＜0.05).AIOD on MASSON coloration: Compared with Group CTRL, Group BLM, Group CH,Group PF and Group CP have higher AIOD(P＜0.01), which mean much more Collagendeposition on alveoli. Compared with Group BLM, Group CH, Group PF and Group CPhave fewer deposition inferred by lower AIOD(P＜0.01). Group CP has lowest AIODamong Group BLM, Group CH, Group PF and Group CP(P＜0.01).Western blot on ALK5: Group BLM, Group CH, Group PF and Group CP expressionmuch more ALK5to Group CTRL(P＜0.01). Compared with Group BLM, Group CH,Group PF and Group CP have lower expression(P＜0.01), and Group CP has an evengreater reduction than Group PF(P＜0.01) as well as Group PF and Group CH have in-significant variance(P≥0.05).Western blot on Smad2/3: Compared with Group CTRL, the expression of Smad2/3in-creases in Group BLM, Group CH, Group PF and Group CP(P＜0.01) which also de-creases compared to Group BLM(P＜0.01). But Group CH, Group PF and Group CPhave little significant difference among them (P≥0.05).Western blot on EGFR: Compared with Group CTRL, Group BLM, Group CH, Group PFand Group CP have significant higher expression of EGFR(P＜0.01), and Group CH,Group PF and Group CP decreases the expression to Group BLM(P＜0.01). But the ex-pressions of EGFR in Group CH, Group PF and Group CP reduce a similar level(P≥0.05).Use TBA method assay MDA: the content of MDA in Group BLM, Group CH, Group PFand Group CP is much more than Group CTRL(P＜0.01), and Group BLM is2.5foldthan Group CTRL. Group CTRL and Group CP are in the same level (P≥0.05). Comparedwith Group PF, Group CP has less content of MDA(P＜0.05).ConclusionHuoxue huayu prescription can treat bleomycin induced pulmonary fibrosis in rats.Moreover, combination of Huoxue huayu prescription and pirfenidone can improve the Treatment outcome.Huoxue huayu prescription may decrease the expression of ALK5,Smand2/3and EGFR, suppress MDA, and play multi-approach and multi-target an-ti-fibrosis effects. ObjectivesTo explore the clincal features and radiological pattern of interstitial lung disease, im-prove the awareness of ILD, and serve the diagnosis and treatment.MethodsA total of21interstitial lung disease patients who were hospitalized in First AffiliatedHospital of Dalian Medical University during August2013to December2013wereincluded in this retrospective study. Investigate their clinical characteristics such assymptoms, physical signs, radiography features and arterial blood gas analysis.ReslutsThere are mainly old patients in this study. COPD combined ILD, idiopathic pulmo-nary fibrosis and connective tissue disease combined ILD account for a large ratio ofILD, and other causes are relatively infrequent.Most patients have cough and dyspnea,while the Velcro rales are frequently auscultated. The most common radiological pat-tern of ILD is reticular opacities and often seen at subpleural and paraseptal areas ofthe lower lobe. Lung fuction suggest that most patients have mixed mixed ventilationdysfunction with low diffusion capacity for carbon monoxide of the lung.ConclusionMost interstitial lung diseases have typical clinical features. Doctors should improvethe knowledge of interstitial lung disease, improve the auxiliary test and improve theeffect of diagnosis and treatment.