The Expression Of Th17Cells In Rats With Renal Impairment Reduced By Salt Sensitive High Blood Pressure

[Objective] The aim of this research is to investigate the expression of Thl7cell in rats with kidney damage reduced by DOCA--salt sensitive high blood pressure and the effect of Mycophenolate Mofetil(MMF) on cell regulation.[Methods]24male healthy Wistar rats were randomly divided into three groups after receiving nephrectomy on the left side kidney:the control group (were injected subcutaneously with vegetable oil; drink tap water and rats stomachs were perfused with saline water); the model group (were injected subcutaneously with DOCA; drink1%Nacl0.2%KCL saline and rats stomach were perfused with saline water); the MMF group (were injected subcutaneously with DOCA; drink1%Nacl0.2%KCL saline and rats stomach were perfused with MMF).The rats were put to death after eight weeks. We adopted Flow Cytometer to test what percentage of Th17cells in CD4+T cells in peripheral blood and used ELISA to test IL-17level in serum. The change of transcription factor expressed protein ROR-yt in kidney tissue was detected by western blot. Semi-quantitative analysis of renal pathology injury;24hour urinary protein (Upro) was assayed.[Results] The numbers of Th17cells increased in rats with kidney damage reduced by DOCA--salt sensitive high blood pressure and the expression of IL-17and ROR-γt,Upro increased significantly. Kidney histological damage is serious of the model group compared with the MMF group.[Conclusion] The expression of Th17cells increased and may play a part in the course of renal impairment reduced by DOCA--salt sensitive high blood pressure. MMF can relive Th17cell infiltration in kidney tissue and immunosuppresive therapy may protect the kidney damage from the hypertension...