Impact Of Alendronate Combined With Simvastatin On Osteoporotic Fractures Healing

Objective：To investigate the effects of alendronate combined with simvastatin onthe osteoporotic fracture healing in rats．Methods：50SD adult female rats were randomly divided into5groups:（A）shamoperation control group;(B)ovariectomized control group;(C)ovariectomizedalendronate group;(D)simvastatin group;(E)ovariectomized alendronate andsimvastatin group.B，C，D,Egroup underwent ovariectomy surgery, A group onlypartially excised adipose tissue.8weeks later,2rats were selected randomly from eachgroup to confirm osteoporosis successful modeling.Then the midshaft femur fracturemodel was established by an operation and intramedullary nails fixation was achievedwith Kirschner wire.Deal each group of rats with such drug intervention as follows:(A,B) physiological saline5ml/kg/d gavage for8weeks;(C)alendronate0.5mg/kg/dgavage for8weeks;(D)simvastatin20mg/kg/d gavage for8weeks (E）alendronate0.5mg/kg/d+simvastatin20mg/kg/d gavage for8weeks. All the rats were killed andtheir blood samples and left femurs were collected.The serum calcium, phosphorus,alkaline phosphatase(ALP) content,X-ray bone mineral density(BMD) andbiomechanical strength were detected.Results: Compared with the sham group, the ovariectomized group hadsignificantly reduced bone mineral density, elevated ALP level and serum calcium,phosphorus content had no significant change.After treatment witn alendronate andsimvastatin for8weeks, calcium, phosphorus content had no significant change；alkaline phosphatase level decreased；femoral bone mineral density and biomechanical strength were improved.This result is more obvious in alendronate andsimvastatin group, the difference was statistically significant.Conclusion: Alendronate is beneficial to the restoration of biomechanical functionin the osteoporosis fracture healing, and simvastatin can play a synergistic effect.