Effect Of SLIT2Gene Transfection On Vascular Endothelial Growth Factor In Retinal Pigment Epithelium Cells And Its Mechanism

Objective：To investigate the effect of Slit2gene on vascular endothelial growthfactor（VEGF）in retinal pigment epithelium（RPE）cells and the functionof PI3K/AKT and MEK/ERK signaling pathways during the course．Method：human APRE-19cells were cultured in vitro.2. RPE cells transfecteby adenovirus-mediated Slit2(Ad-slit2),then culturaled12h、24h、48h.3.Before transfected by Ad-slit2,RPE cells were pretreated with MEKinhibitor PD98059、PI3K inhibitor LY294002for1h, then cultured12h、24h、48h.4.VEGF mRNA expression levels were detected by quantitativePCR and the protein expression levels were detected by ELISA.The proteinexpression levels of t-ERK、t-AKT、p-ERK、p-AKT were detected byWestern blot.Results： VEGF mRNA and protein expression levels were obviously increasedin RPE cells transfected with Ad-slit2at48h（P<0.05），p-ERK、p-AKTprotein expression levels were increased in RPE cells transfected withAd-slit2（P<0.05），but t-ERK and t-AKT remained unchanged，PD98059decreased the activation of p-ERK and LY294002decreased the activationof p-AKT，VEGF mRNA and protein expression levels were decreased byPD98059and LY294002（P<0.05）.Conclusion:The expression of the VEGF in RPE cells transfected with Ad-slit2was increased.by MEK/ERK and P13K/Akt signaling pathways.