Expression And Function Of Several MicroRNAs In Myeloid Leukemia And K562Cells

Expression and function of several microRNAs in myeloid leukemia and K562cellsBackgroundIn recent years, microRNAs became a hot research field of molecular biology, and caused increasingattention of the masses of scientific workers especially its relationship with the tumorigenesis anddevelopment. microRNAs widely exist in almost all kinds of mammal creatures. But so far, of all thehundreds of microRNAs, only a small number of microRNAs have been systematically studied. With thedeepening of the research on microRNA, scientists found that the microRNA mutation or abnormalexpression is associated with a wide variety of tumor development, and different microRNAs in differenttype of tumors are not consistent. Increase of some microRNAs expression or mutation can promote cellcarcinoma gene expression, and thus cause the malignant cell transformation. Measurement of microRNAthereby may be used in the diagnosis and prodiction of cancer treatment.At present, Northern blot and chip can be used to determine the tissue specificity of microRNAsexpression in specific organs. Detection of unique microRNAs expression spectrum can be classified on thetumor, and determine wether can be used to estimate the prognosis of patients with microRNAs tag.Therefore, according to a wide variety of tumor microRNAs expression spectrum feature library, data canhelp in the diagnosis and treatment based on tumor specific microRNAs expression. And expressiondifferences have been shown to helpfully predict the prognosis of patients. Therefore, microRNAexpression levels may provide a powerful tool to guide clinical treatment. At the same time, microRNAsexpression profiles for elucidating disease stages and mechanism provide a new treatment strategy and willalso play a particularly important role in clinic.Leukemia is hematopoietic stem cell cloning of disease, is a set of highly heterogeneous malignancies.Leukemia characteristic mainly display cells with abnormal proliferation and differentiation mature barriersand apoptosis inhibition. At present, the cause of leukemia is not fully clear. Many factors related to itspathogenesis and the virus may be the main factors. In addition, there are comprehensive factors such asphysical and chemical genetics. With the progress of molecular biology, the etiology of leukaemia becomes an important research field of molecular biology. Increasing evidence has shown that expression of certainmicroRNAs is aberrant in leukemia and involves in deregulation the expression level of downstreammolecules, especially transcription factors. Based on current literature, microRNA expression is aberrant inmyeloid leukemia, but the specific expression profiles and their target molecues are unclear.MethodsThis study analyzed the clinical documental cases combining leukemia cell lines to study the effect ofseveral microRNAs in myeloid leukemia development and progression. Real Time-PCR was used for thedetection of several microRNA expression levels in patients with myeloid leukemia in the bone marrow.Cell transfection experiments were used to observe the effects of the microRNAs on K562cellularbiological behavior. Trypan blue staining and MTT assay were used for detection of the cell proliferationability. Western Blot technique was employed to detect the level of cell differentiation protein markerMyadm.ResultsResults of Real Time-PCR showed that the expression of microRNA-127and microRNA-154significantly increased and expression of microRNA-185and microRNA-17-3p decreased in patients withmyeloid leukemia compared to normal controls. When K562cells were transfected with mimicmicroRNA-127and microRNA-154, proliferation of the cells were significantly promoted and celldifferrention related marker Myadm mildly increased. In contrast, transfection of mimic microRNA-185and microRNA-17-3p has inhibited the cell proliferation and has not effects on cell Myadm expression.ConclusionsMicRNA expression and myeloid leukemia development may be related. Expression of micrornas-154and microRNA-127increased in patients with myeloid leukemia, and significantly promote theproliferation of K562cells. The expression of microRNA-185and micrornas-7-3p reduced in patients andinhibited K562cell proliferation with transfection. The microRNA-127and-154anhance Myadmexpression but mechanism of inducing cell differentiation remained to be elucidated.