The Effects Of Gingko Flavonoid On The Expression Of NF-κ Bp65in Mice With Non-alcoholic Fatty Liver Disease

Backgroudn and Objective: The incidence of Nonalcoholec fatty liverdisease(NAFLD) in the general population has increased year by year in recentyears, and it’s prevalence rate reached15%in Shanghai,Guangdong and otherdeveloped areas. Some patients may develop into nonalcoholic steatohepatitis,cirrhosis, and even liver cancer,thus it increasingly cause the attention ofpeople. The pathogenesis of NAFLD has not been entirely clear, but theimbalances of inflammatory cytokines is one important link. Tumor necrosisfactor alpha(TNF alpha) is one of the major inflammatory mediators, nuclearfactor kappa-binding (nuclear factor-κB) is the transcriptional regulation factorof inflammation, and they both are closely related to inflammation. Current theresearch on the role of TNF alpha in NAFLD injury is relatively common butnuclear factor kappa-binding is rare, and this subject will study the role of themboth in NAFLD. The gingko flavonoid is the extract of gingko leaf, and in recent years, research has shown that it has the role of anti-inflammatory,antioxidant and regulating metabolism. The therapeutic effect of gingkoflavonoid in NAFLD is discussed by observing the effects of different volumegingko flavonoid on the expression of nuclear factor-κB and TNF alpha inmice with NAFLD in this paper.Methods:120KunMing mice were randomly divided into the controlgroup30only and the high-fat model group90only after being fed one week.Control group were fed with normal diet. High-fat model group were fed withhigh-fat diet(2%cholesterol+5%egg yolk powder+10%lard+0.2%propylthiouracil+72.8%common feed) and that was constructed to the animalmodel of NAFLD in mice. Each group of10mice were executed after4weeksand high-fat model was determined by observing the pathological slices of liver.After the success of building high-fat model the remaining20normal groupcontinued to be fed with normal diet,and20mice that were randomly selectedfrom among high-fat model continued to be fed with high-fat diet and the rest60high-fat model mice were randomly divided into A group，B group and C group.A and B,C three groups also continued to be fed with high-fat diet and weregiven different doses gingko flavonoid to fill the stomach. The dose arerespectively75mg/kg.d,150mg/kg.d,300mg/kg.d.The control group andhigh-fat model group were treated with normal saline to fill the stomach. All themice were executed after16weeks and the expression of nuclear factor-κBp65,TNF alpha, triglycerdes(TG) was respectively detecede with RT-PCRmethod, ELISA method and enzymatic method.Results: The mice with non-alcoholic fatty liver disease were succeededto build by being fed with high-fat diet. Compared with model control group,thelevels of nuclear factor-κBp65,TNF alpha and TG of high-fat model group wereincreased and had statistical significance(p<0.05). Compared with high-fatmodel group,the levels of nuclear factor-κBp65,TNF alpha and TG of A and B,C three group were decreased and had statistical significance(p<0.05),and thehigher dose,the more obvious. Compared between A and B,C group,the resultsalso had statistical significance(p<0.05).Conclusions: The mice with non-alcoholic fatty liver disease can bebuilt successfully by being fed with high-fat diet.Nuclear factor-κB that is thetranscriptional regulation factor of inflammation and TNF alpha that is one ofthe major inflammatory mediators are closely related to the onset of NAFLD.Gingko flavonoid may decrease the levels of nuclear factor-κB,TNF alpha andTG of mice with NAFLD, and the higher dose be gived,the more obvious ofdecrease and it showed that gingko flavonoid has certain therapeutic effect forNAFLD.