Study Of Neurprotective Effect Of Icaritin(ICT)on Aβ42Transgenic Drosophila Model And Its Mechanism Associated With Alzheimer’s Disease (AD)

Objective:To investigate the neurprotective effects of Icaritin (ICT) to Alzheimer’sdisease(AD) on Aβ42transgenic drosophila model related to Alzheimer’sdisease(AD) and the underlying mechanism, aims to provide new choose for thetreatment of Alzheimer’s disease.Methods:1. Established the Aβ42transgenic Alzheimer drosophila model,throughphenotypic observation and HE tanning method to evaluate if the model wassuccessfully estabilished.2. By observation of phenotypic,investigate the effect of ICT on lifespanand climbing abiltity of Aβ42transgenic drosophila model.3. Use biochemical methods to study the effect of ICT about SOD activityand MDA content on Aβ42transgenic drosophila brain tissue homogenates,toresearch if ICT has the oxidation resistence.4. In order to reserch the effect of ICT on the ATP level in Aβ42transgenicdrosophila brain tissue,we used Reversed Phase High-performance LiquidChromatography(RPHPLC) technique. 5. By biochemical methods,detection if ICT can effect the CHE activity onAβ42transgenic male and female drosophila brain brain tissue,preliminaryexplore its nerve protection mechanism.Results:1.Through behavioral observation found that male and female drosophilamodel crawing movement ability were lower and lifespan were shorten.HEtanning results show us that25d male and female drosophila model brain tissueslices nerve cells left a large number empty compared with control group.TheAβ42transgenic drosophlia model was successfully estabilished.2. ICT(1nmol/L) can extending the Aβ42transgenic drosophila’s lifepanand improving the climbing ability.3. Biochemical indicators showed that: Compared with normal flies, Aβ42transgenic Drosophila brain tissue SOD activity decreased, MDA contentincreased; by feeding ICT, compared with the model group, male flies SODvalues improved25.28%, female flies SOD increase11.34%; male flies MDAvalues decreased68.75%, female flies reduced47.05%.4. Reversed Phase High-performance Liquid Chromatography(RPHPLC) technique was used to found that at25days ATP content in Aβ42transgenic drosophila brain was lower than normal drosophila. But thedrosophlia treated with ICT,ATP content was higher than Aβ42transgenicdrosophila,male flies improved36.26%, female flies improved26.70%。5. CHE activity was measured to show that ICT does not reduce Aβ42transgenic Drosophila CHE vitality, the difference does not have statisticallysignificant. Conclusions:1. Aβ42transgenic drosophila model was successfully constrcted.2. ICT(1nmol/L)can extending the Aβ42transgenic drosophila’s lifepanand improving its climbing ability,has certain nerve protective effect.3.ICT can improve Aβ42transgenic male and female flies of SOD, MDAcontent was reduced, thereby enhancing the ability of anti-lipid peroxidationcapacity and free radical scavenging.4.ICT can act on the function of mitochondria through increasing the brainATP content in Aβ42transgenic drosophila.5.ICT(1nmol/L)has no effect on Aβ42transgenic drosophila, itsneuroprotection mechanisms may through improve antioxidant capacity andsupply of energybrain tissue,not estrogen-like effects.