Inhibitory Effects Of Biological Thermochemotherapy On Human Hepatocellular Carcinoma HepG2Cells In Vitro

Background and purpose:Liver cancer is one of the most common malignant tumor in our country. Inmalignant tumor,the incidence of liver cancer worldwide each year was fifth,mortality has been ranked second,and onset is insidious, development is rapid,invasiveness is strong,recurrence rate is high, treatment is more difficult,whendoctor has middle late, and only10%~20%can be surgically removed,the mediansurvival is only3~6months., There are over20million people died of liver cancerworldwide each year,known as "the king of cancer". The middle-late liver cancer aretreated with high-dose chemotherapy, radiotherapy, radio frequency, microwave,high intensity focused ultrasound, laser, and interventional therapy, but theimprovement in survival is still very limited,long-term effect is poor, andeffectiveness and side effects can not be taken into account.Currently there is nostandard treatment.So to find a safe and effective method of cancer therapy hasbecome a new research focus.Biohyperthermia,biochemot-herapy and thermo-chemotherapy are the new cancer treatment methods, andhad a very good therapeutic effect on tumor. In recent years,the studies found that the development of malignant tumors was not just from the unlimited proliferationcells,the cell apoptosis mechanism barrier was also a major cause of tumoroccurrence and development. Thus inducing apoptosis research has become ahotspot in recent years.Biohyperthermia, biochemotherapy and thermochemotherapyall have the killing effect on tumor,but the present study is limited to a single,combined effect of the three studies at home and abroad has not been reported. Thisexperiment is designed to study the effect of bio-thermochemotherapy on theproliferation and apoptosis of human hepatocellular carcinoma cells,and provides anew way of thinking and method for the treatment of liver cancer.Methods:1. Human hepatocellular carcinoma cells HepG2as the research object, usingthe water bath heating method.Cell were subjected to thermotherapy at43℃for60min and observed the proliferation and apoptosis after24h of treatment.2. The cells growth inhibition effect was evaluated by MTT assay.3. Synergy q value was calculated to understand the nature of the interaction ofbiohyperthermia,biochemotherapy and thermochemo-therapy.4. The cell cycle and apoptosis rates of HepG2were determined by flowcytometric analyses.5. Analyzed the activity of caspase3by fluorescence intensity.6. Statistical analysis used SPSS17.0statistical software.Results:1.The proliferation and apoptosis rate of bio-thermochemotherapy wassignificantly higher than the other groups.2.Combination of the three made cell cycle simultaneously arrest at G0/G1andG2/M phase and S phase was significantly decreased.3.ADM in combination with sorafenib on the basis of hyperthermia haveadditive effect on killing HepG2cells.4.The activity of caspase-3of bio-thermochemotherapy significantly enhancedthan the other groups.Conclusion:Biohyperthermia,biochemotherapy and thermo-chemotherapy can obviously increase the inhibition of proliferation and the apoptosis rates of HepG2cells. Thecombined effect of the three was significant compared to separate effect,and theeffect was shown as additive effect.The mechanism may be related to cell cyclearrested and caspase-3activity increased.