| Background and objective:With the development of modern medical technology extending the human lifespan gradually, the incidence of atherosclerotic disease is also rising. Followed arterial ischemic disease. Now, there are many ways to treatment arterial ischemic disease. For example:The fogarty catheter embolectomy and catheter balloon dilation, etc. Although it can quickly restore blood supply, but at the same time it can also lead to "ischemia-reperfusion injury(IRI)", It caused the patient prognosis slip and reduces the clinical success rate of treatment. At present, the focus of basic and clinical research is to how to avoid or reduce IRI’s influence on the prognosis of patients. The current research focuses on the vital organs of the human body (such as the brain, heart, lung, etc.), but few studies of skeletal muscle IRI. As a main power organs skeletal muscles’ tolerance is lower than the other organs of ischemia. When restoring tissue perfusion, IRI is easier to happen. So, the study of skeletal muscle IRI is particularly important. In this study, through the establishment of the rabbit hindlimb ischemia-reperfusion model to investigate the protective effect of hypothermia on IRI, so as to provide new avenues of prevention and treatment of ischemia-reperfusion injury. Method:36adult rabbit, no matter male and female, weighing between2-2.5kg. They were randomly selected into A, B, C, D, E, F6group (n=6). Experimental subject’s are prohibit eating and drinking12hours before surgery. In the abdominal cavity5ml/kg bolus of3%chloral hydrate anesthesia. After the success of the anesthesia, fixed the animals on the operating table with supine position. Line up the femoral artery cut the skin of about10cm long. Reveal the shares of the neurovascular bundle, free the femoral artery about5cm. Use the non-invasive vascular clamp clip the femoral artery. With placement in the femoral artery and femoral vein and femoral nerve at the bottom of the homemade hemostatic blocked artery collateral circulation.and at the same time keep the femoral vein reflux smooth.Set group A to blank group, group B for IRI, group C for local temperature10℃group after reperfusion, group D for local temperature15℃after reperfusion, group E for local temperature20℃after reperfusion, group F for local temperature25℃after reperfusion. Vascular exclusion time is4hours. After reperfusion the above A, B, C, D, E, F groups respectively proceeded right atrium puncture haemospasia in1hour,2hours,4hours,8hours,12hours respectively. Automatic biochemical analyzer to detect serum creatine kinase (CK), lactate dehydrogenase (LDH) level. Enzyme-linked immunosorbent assay (ELISA) method for determination of serum interleukin6(IL-6) expression level. At4hours, take the hind limb ischemia-reperfusion posterior tibial muscle tissue about0.6g. Wherein cut0.3g, placed in a test tube filled with formalin solution, HE dyeing, used to observe the fine structure of the muscle tissue changes. The remaining muscles used to detect muscle wet-to-dry ratio (W/D).Results:1. After reperfusion serum LDH, CK, IL-6level comparison.4hours after reperfusion, serum LDH, CK level statistical comparison:group A at each time point was no significant difference (P>0.05). Group C, group D and group E compared with group B levels decreased, there are statistically significant differences (P<0.05). Each time point in group F compared with group B levels rose (P<0.05). No significant difference in IL-6levels at each time point by statistical comparison of group A (P>0.05). Group B at each time point level are higher than the other groups, to achieve the highest value of41.50±4.59at4hours. Group C, group D, E and F group at each time point compared with group B have different degrees of decline (P<0.05).2. Comparison of muscular W/DAfter4h reperfusion, compared with group A, there is different degree of edema in the other group of muscles (P<0.05).The muscle W/D was4.78±0.87in group C, which is the lowest of all the experimental groups(except group A). The muscle W/D was6.07±0.63in group F, that is the highest of all the experimental groups(except group A). Muscle edema degree is the most serious. The difference was significant statistically significant.Conclusion:Hypothermia has significant protective effects in rabbit skeletal muscle IRI. The local temperature of10℃is the strongest protective temperature, at which protection is achieved by reducing reperfusion tissue inflammation. |
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