| Arrhythmia means the abnormality of the heart impulse, rhythm, origin place,conduction velocity or excited order. As a common clinical cardiovascular diseases orcomplication, clinical symptoms are preformed in different ways. The lethalarrhythmia is a major cause of sudden death, which seriously damages the patient’shealth. The main treatment of arrhythmia still is drug treatment, but the medicationused to treat arrhythmia can also cause irregular heart rhythm. Researches of themedication, which can intervene multiple targets and restore normal physiologicalfunction of the ion, will be future direction of the drug investigation. Cassia twig andmain licorice decoction was documented in ‘Shang Han Lun’, which is a basicformula to treat palpitation. Cassia twig and main licorice decoction is helpful foranti-arrhythmic, but its anti-arrhythmic mechanism is unclear. This experiment usedmodern separation method to extract the effective part of compound. On a macro scale,it reflects the fingerprint method of material group in Chinese traditional medicine,and clarifies the formula compatibility of cassia twig and main licorice, componentsand the change of traditional Chinese medicines with serum. After we identify theeffective components of Cassia twig and main licorice decoction, it will be theprerequisites for further electrophysiological mechanism researches.Objective: Quality control for the single medicinal materials of cassia twig and mainlicorice. Identify the formula compatibility of cassia twig and main licorice. Evaluatethe material group composition and change of traditional Chinese medicines withserum.Methods: Using HPLC to establish a method of high sensitivity, good stability andaccuracy of guizhi soup licorice extract ingredients categories were studied.By LC-MS clear cassia twig water extraction liquid, main licorice water extraction liquid,guizhi soup licorice extract the main composition of drugs and change.Of serumcollected by LC-MS clear the main ingredients, compared with the corresponding groups of drugs in vitro fingerprint comparative analysis, identify ownership comesfrom various ingredients composition peak, confirm the prototype components andmetabolites, tracking the effects of the compatibility of traditional Chinese medicineand formula composition.Results:1, this study established the cassia twig, main fingerprint, liquorice, cassiatwig licorice soup by comparison with traditional Chinese medicine (TCM)fingerprint similarity evaluation system shows that there are four common peak of10batches of cassia twig, main licorice has16common peak, cassia twig with25common peak in liquorice decoction, a total of10batches of herbs through contentdetermination component content difference is bigger.2,By using the method of cassiatwig fingerprint chromatography of cassia twig to evaluate the stability of waterextraction liquid, found the main cinnamic acid in cassia twig peak area of RSD21.51%within10h,6h peak area in the RSD was2.77%.3,the method of using cassiatwig licorice soup fingerprint chromatography conditions on the stability of guizhisoup licorice extract, results within12h licorice glycosides peak area RSD was1.71%,RSD was1.29%, cinnamic acid peak area cinnamic aldehyde peak area RSD was2.20%, glycyrrhizic acid glycosides peak area RSD was0.58%.4, using liquid massspectrometry is concluded cassia twig, main licorice and cassia twig liquoricedecoction of main chemical composition of molecules, cassia twig to identify85prospective molecular ion peak, a main licorice identified28quasi molecular ion peakand guizhi soup of licorice identified98quasi molecular ion peak, and compare thecassia twig and main licorice according to the proportion of treatise on febrile diseaseguizhi liquorice decoction prescription compatibility after new chemicals, a total of45.5, using liquid mass spectrometry comparing chemical molecules in vitro and invivo transitional ingredients that guizhi has eight chemical composition molecules areabsorbed into the blood, in the main licorice has5chemical molecules are absorbedinto the bloodstream, cassia twig licorice soup with12chemical molecules areabsorbed into the blood,Composition of cassia twig in vitro with only one commoningredient blood transitional components, cassia twig licorice soup decoction liquidingredients and blood transitional components in common ingredients, cassia twig,cassia twig liquorice decoction serum transitional components in seven commoningredients.Conclusions:1, this study established the cassia twig, main licorice, cassia twigliquorice decoction of fingerprint, chose the optimal chromatographic conditions and detection wavelengths, each component chromatographic peak separation degree andthe peak shape is good.Show that the method is of high sensitivity, good stability andaccuracy, through the "traditional Chinese medicine (TCM) fingerprint similarityevaluation system" of2004edition of10batches of cassia twig, main licorice, cassiatwig licorice soup fingerprint analysis, similarity of each sample is good, cassia twig,main licorice, cassia twig liquorice decoction quality standard established providereliable scientific basis.Also found cassia twig, main licorice, part of the commonpeak content in cassia twig licorice soup is put in bigger difference, cassia twig,shows that different sources of the composition of licorice although chemical speciessimilarity is higher, but the content is put in bigger difference, these differences mayaffect the efficacy, so in the application of cassia twig licorice soup, should strictlycontrol the sources of the raw material slices and quality.2, the main ingredient incassia twig cinnamic aldehyde easy oxidation in air, and stability is poorer, cassiatwig liquorice decoction applied when the storage time could affect efficacy.3, cassiatwig and grilled liquorice decoction, cassia twig decoction is obviously better than thestability of cinnamic aldehyde.4, cassia twig obtained by liquid mass spectrometry,acupuncture is the main chemical composition of licorice and cassia twig licorice soupguizhi liquorice decoction and decoction with single decoction liquid ratio, a newchemical composition.5, through the liquid mass spectrometry is concluded cassiatwig outside the single decoction liquid composition compared with blood transitionalcomponents, found a common ingredients, can be directly absorbed into the bloodcomponents of liquids.6, through liquid mass spectrometry is concluded cassia twiglicorice soup decoction liquid ingredients and blood transitional components notfound in the common elements, may give medicine drug metabolism of eachcomponent has occurred after1hour.7, through the liquid mass spectrometry isconcluded cassia twig, cassia twig liquorice decoction serum transitional componentsfound in seven common ingredients, probably from cassia twig into the compositionof blood metabolites. |
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