| In the thesis, high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) was used in analyzing pesticides residue in TCM; Capillary electrophoresis (CE) was applied in the separation of chiral pesticides. Furthermore, chiral separation mechanism was preliminarily investigated. The main content of the thesis are as follows:1. The recent advances on the analytical methods for pesticides residue in TCM by chromatography coupled with mass spectrometry methods, sample pretreatments and chiral separation areas were summarized.2. A method of HPLC-MS for the simultaneous determination of23pesticides in Traditional Chinese Medicine Paeoniae radix Alba was developed. Recoveries between70%and120%were obtained for all pesticides at0.01mg/kg and0.1mg/kg levels, except for polar pesticides such as thiamethoxam and imidacloprid. Limits of quantification (LOQs) were between0.75～12.17μg/kg. Twenty four batches of Paeoniae radix Alba samples from Zhejiang, Jilin, Sichuan, Anhui and Henan were analyzed.3. A new method of capillary zone electrophoresis (CZE) was established for four chiral pesticides, imazaquin, imazamox, clethodim and tepraloxydim. The effects of chiral additive concentration, buffer concentration, and pH on chiral separation were investigated. Thermodynamic parameters of chiral separation process were measured. Chiral separation mechanism was preliminarily investigated.4. A new method of micellar electrokinetic capillary chromatography (MEKC) was established for four chiral pesticides, flutriafol, triticonazole, etoxazole and epoxiconazole. The effect of chiral additive concentration, buffer concentration, pH, surfactant type, surfactant concentration, organic modifier concentration, sample solvent and temperature were investigated. Thermodynamic parameters of chiral recognition process were measured. The possible chiral recognition mechanism was deduced. |
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