Anti-Inflammatory Effect And Regulation On MAPKs Signal Transduction Pathway In Vitro By Taraxasterol

Objective:We used traditional taraxasterol isolated from Herba Taraxaci as object, and studied anti-inflammatory effects and its mechanism in vitro.Methods:The experiment divided into blank groups, model groups, taraxasterol groups (low, medium and high). Anti-inflammatory system was established in vitro through the mouse peritoneal macrophages induced by LPS. Cell toxicity was measured by MTT method, and dose was determined. NO concentration was measured by Griess method. PGE2concentration was measured by ELISA method. iNOS and COX-2mRNA expressions were determined by RT-PCR method. COX-2and iNOS protein expressions were determined by Western blot method, and determined the phosphorylation levels of ERK, JNK, p38protein expressions.Results:1. The anti-inflammatory of taraxasterol test showed that was decrease significantly comparing with model groups, the degree of inflammation taraxasterol groups (low, medium and high), taraxasterol significantly inhibited the release of NO and PGE2in RAW264.7cells; taraxasterol of different dose inhibited the expression of iNOS, COX-2mRNA and proteins in a dose-dependent relationship.2. The mechanism of anti-inflammatory test showed that taraxasterol inhibited the phosphorylation of p38, ERK1/2and JNK kinases in mouse RAW264.7cells induced by LPS, in a dose-dependent relationship.Conclusion:Taraxasterol inhibit the expression of INOS, COX-2mRNA and proteins by inhibiting MAPK, p-ERK1/2and p-p38pathway of the RAW264.7cells induced by LPS, and reduce the release of inflammatory mediator of the cells.